HEMATOLOGIC AND MOLECULAR GENETIC STUDIES OF ANIMAL BLOOD FOR TESTING PREPARATION " LEYKOZAV " AGAINST THE VIRUS BOVINE LEUKEMIA
DOI:
https://doi.org/10.31548/dopovidi2016.04.023Keywords:
leukemia, diagnosis, hematology, ELISA, PCR, cattleAbstract
This paper presents the results of research in the blood of cattle bovine leukemia. Leukemia cattle - retroviral etiology of tumor disease which has major damage to livestock. Studying the problem of leukemia, scientists have come to a common conclusion - for active struggle must create immunogenic drug against leukemia.
The data of experimental work carried out in conditions of epizootic experiments are new and will be able to assist the practical and scientific veterinary experts in carrying out measures to combat this dangerous disease of animals and humans. For this purpose, we developed a new technology for production of inactivated immunogenic preparations, including against bovine leukemia, which the cows tested showed positive results.
Employees " State Centre of Innovation Biotechnologies " offered new innovative manufacturing techniques inactivated, immunogenic, harmless drug "Leykozav" which received positive results in the laboratory and epizootic experiments. The drug has not only specific protective properties, but also a positive impact on gender-positive animals (experiments were 50-70% recovery).
Objective- to investigate the incubation period of the cow leukemia in terms of epizootic experiment using RID, ELISA and PCR. Influence of "Leykozav" the population of virus in the body and sick cows on hematological parameters of experimental animals.
Materials and methods. The study was conducted in disadvantaged on leukemia cattle farm "Danivka" Kozelets district, Chernihiv region, with the number of dairy cattle in 460 cows. Before the experiment, blood and serum of animals examined by RID, ELISA, PCR and hematologic.
To study the effect of post-vaccination immunity in a population of virus in the blood of sick cows was formed group (n = 6) who were administered the drug "Leykozav" subcutaneously at a dose of 4 cm3 twice with an interval of 21 days. Cows in the control group were administered saline (0,9% NaCl) by a similar scheme.Blood samples for the study were collected before the experiment quarterly and nine months (period of observation).
Results and discussion. The first symptoms of leukemia among healthy cows (control group), are among the troubled animal herd, appeared after 6 months of the experiment. This is confirmed by real-time PCR.Immunization RID-positive animal drug "Leykozav" impact on reducing the number of copies of DNA provirus leukemia. After the drug decreased the number of copies 7-420 times.
The animals in the control group during the period of the experiment marked increase in the number of white blood cells by 34% relative to the norm, whereas other hematological parameters: red blood cells, hemoglobin, hematocrit and platelets remain in the normal range.
The use of a series of experimental drug "Leykozav" sick cows leukemia research farm led to minor hematological changes. WBC early experiment was 17,05 ± 3,76, after 9 months. experiment - 15,72 ± 3,37. There is a tendency to decrease. In this case, the concentration of white blood cells decreased by 15.7%. Indicators of red blood cells, hemoglobin, hematocrit and platelet counts in vaccinated animals did not significantly change during the research period and are in the normal range. Thus, the drug does not cause adverse changes in the blood of animals after immunization of prophylactic drug "Leykozav."
Conclusions and prospects. 1. In healthy animals that are disadvantaged on leukemia studies, development of infection registered in the 6-9 month studies confirmed that ELISA and PCR.
2. Positive reaction by RID is on the ninth month of the disease, the animal stays in disadvantaged on leukemia herd.
3. Using the quantitative determination of leukemia provirus DNA using polymerase chain reaction not only can detect infected animals in the earliest stages of the disease, but also enables the future and assess the effectiveness of drugs that are recommended for the prevention and treatment of leukemia cattle.
4. Preventive immunization of patients with leukemia drug cow "Leykozav" reduces the number of copies of DNA provirus in blood in 7-420 times.
5. To maintain stability on the welfare of cows in the herd leukemia recommend scheplyuvaty all animals once a year, in the spring or autumn, the drug "Leykozav", following the rules of aseptic and antiseptic.
References
Yarchuk B. M., Trsyn R. V., Dovhan' O. V. (2013) Epizooticheskaya situatsуya y organizatsionno-metodicheskуe osnovy ozdorovlenyya ot leykoza khozyaystv Ukrainy [Epizootological situation,organization-methodical and molecular-biological aspects of environmental sanitation of large horned cattle from a leukosis in Ukraine], Scientific Journal of Veterinary Medicine, 12 (107), 83 – 87.
Mandygra M. S. Lyubar N. V., Udyak V. M. (2011) Otsenka prizhiznennoy dyahnostyky leykoza krupnoho rohatoho skota [Еstimation of life diagnostics methods of leucosis of cattle], State Research Control Institute of veterinary drugs and feed additives, 12. № 1, 2, 362 – 367.
Instruktsiya z profilaktyky ta ozdorovlennya velykoyi rohatoyi khudoby vid leykozu [Instructions for the prevention and rehabilitation of bovine leukemia] (2007), State Committee for Veterinary Medicine of Ukraine 13
Busol V. O., Kovalenko L.V., Tons'ka T. H. (2010) Imunohenni kharakterystyky inaktyvovanykh vaktsyn proty leykozu velykoyi rohatoyi khudoby [Immunogenicity characteristics versus inactivated vaccine bovine leukemia], Scientific Journal of Veterinary Medicine, 6 (79), 44–46.
Busol V. A. (1982) Epizootology leukemia cattle. All-Union Conference, Moscow, 18-22 oktоbrе, 191–192.
Busol V. A. Dombrovskyy A. B. (1987) Еpyzootycheskyy protsess pry leykoze krupnoho rohatoho skota [Epizootic process in leukemia cattle] Theoretical and practical issues in veterinary medicine, 28–29.
Hulyukyn M. Y. Zamaraeva N. V. (1994) The study of the level of danger of the animal in a latent stage of infection, as a source of virus bovine leukemia. Recent advances in the fight against and prevention of bovine leukemia: Mes. Rep. Scientific. Manuf. Conf. Kishinev, 15-18 September 15–16.
Mandуhra N. S. (1990) Theoretical and practical basis of the fight with leukemia cattle. The problem of improvement of farms from bovine leukemia. All Union. Scientific-Manuf. Conf., Novosibirsk, 11-13 april, 27–28.
Shykov A. T., Andriyan E. A, Avanensov R. A. (1972) V kn. Vsesoyuz. sympozyuma po probleme leykoza sel'skokhozyaystvennуkh zhyvotnуkh [Union in the book. Symposium on leukemia farm animals], Moscow, Russia, 24.
Zaviryukha H. A. (2009) Profilaktychna imunizatsiya koriv vaktsynoyu «Leykozav» – osnova ozdorovlennya ta aktyvnoy borot'by z leykozom velykoyi rohatoyi khudoby [Preventive immunization of cattle vaccine "Leykozav" – the foundation of health and active fight against bovine leukemia]. Veterinary Biotechnology Newsletter, 14, 103–111.
Zaviryukha H. A. (2010) Formuvannya imunitetu u koriv, shcheplenykh vaktsynoyu «Leykozav», v umovakh epizootychnoho eksperymentu ta yoho vplyv na ozdorovlennya vid leykozu [Formation of immunity in cattle vaccinated vaccine "Leykozav" in terms of epizootic experiment and its impact on recovery from leukemia]. Veterinary medicine, 92, 203–207.
Zaviryukha H. A., Zaviryukha A. I. (2010). Vplyv vaktsyny «Leykozav» na eliminatsiyu virusu leykozu v shcheplenykh RID–pozytyvnykh koriv [The impact of the vaccine "Leykozav" on elimination leukemia virus in vaccinated RID-positive cows]. Veterinary medicine, 94, 173–175.
Zaviryukha H. A. (2011) Rozrobka metodyky standartyzatsiyi inaktyvovanykh vaktsyn proty leykozu velykoyi rohatoyi khudoby [Development of methods for standardization of inactivated vaccines against bovine leukemia] / Problems zooengineering and Veterinary Medicine, 23 (1), 169–172.
Zaviriukha H. A., Zaviryukha A. I. (2011) Novyi fektyvnyy sposib ozdorovlennya velykoyi rohatoyi khudoby vrazhenoyi virusom leykozu [The new efficient way to healing the affected bovine leukemia virus]. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named. S. Z. Gzhytsky, 13 (50), 141–147.
Zaviryukha H. A. (2012) Profilaktychni ta protypukhlynni vlastyvosti vaktsyny «Leykozav» [Preventive vaccines and antitumor properties "Leykozav"].Veterinary medicine, 96, 157–158.
Zaviryukha H. A. (2012) Efektyvnist' profilaktychnykh shcheplen' khudoby vaktsynoyu «Leykozav» v borot'bi z leykozom velykoyi rohatoyi khudoby [The effectiveness of preventive vaccination of cattle vaccine "Leykozav" in the fight against bovine leukemia]. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named. S. Z. Gzhytsky, 14 (53), 55–58.
Kovalyuk N. V., Yakusheva L. Y. (2012) Metody dyahnostykry leykoza krupnoho rohatoho skota [Methods of diagnosis of bovine leukemia]. Krasnodar, 39.
Podуmalkyn A. A. The use of Real-Time PCR for the diagnosis of genetic resistance to cattle leukemia Available at: http: //www.biotech-bryansk.net/nfiles/news/b_A0514E5A-53E6-4F46-B765-DA28B6922049.pdf
Downloads
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
