THE EFFECT OF Nb-CONTAINING NANOCOMPOSITES BASED ON SAPONITE ON THE INDUCTION OF CHLOROPHYLL FLUORESCENCE IN LEAVES OF CORN

Authors

  • M. V. Savchuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. F. Starodub National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi2017.03.004

Keywords:

nanocomposite, photosynthesis chlorophyll fluorescence induction, corn, Kautsky curve

Abstract

Now in Ukraine there is an increased interest in the application of nanotechnology. Nanomaterials in agriculture are used as biostimulants and micronutrients, because they have several advantages compared to traditional means: not stratified under the influence of heat and light, provide full wetting of the plant surface, completely absorbed by the plants does not get washed with rain, the prepared working solution can be stored for years, while remaining active.

Today, nanotechnology opens up great opportunities in agriculture. Nanoparticles are used for presowing treatment of seeds of plants, resulting in its improved quality , increased resistance to phytopathogens and increase the yield of agricultural crops.

The aim of this work was to investigate the influence of new Nb-containing nanocomposites on the parameters of induction of chlorophyll fluorescence in plants of maize (hybrid Kharkivskiy 340 MV).

The objects were 3 types of nanocomposites 1) Saponite; 2) Nb-Sap (EtO); 3) Nb-Sap (Cl). Newly established nanomaterials were provided at NATO project № 984481 NUKR.SFP. The measurements were carried out by a portable fluorimeter "Flora-test" for express-diagnostics of plant state in the early stages of development. Kautsky curves were analyzed and the number of indicators of the induction of chlorophyll fluorescence in plants which were germinated in the presence of nanocomposites and without them (control).

The process of functioning of the photosynthetic apparatus is one of the most vulnerable to stress factors, so significant information about the state of the photosynthetic apparatus of plants under the action of different drugs can be to investigate the method of fluorestsentnogo analysis. During the Kautsky curve analysis identified a number of indicators IFH.

According to the results of investigations it was established that nanocomposites had effect on a number of values of IFH. The value of F0 increased in the processed nanocomposites of maize plants and it was higher compared with the control plants. This indicates an increase of the antenna chlorophylls in the studied samples compared with control as a result of structural changes of the pigment complex of plants of maize for the actions of the nanocomposites. For the actions of Saponite(H), Nb-Saponite(Cl) and Nb-Saponite(Et) on plants value of d Fpl increases compared to the control by 20.8%, 29.2% and 25% respectively. These data indicate an increase in the magnitude of increase of fluorescence from F0 to Fpl in plants by the action of nanomaterials.

The efficiency of photochemical energy conversion in PS II was calculated according to the formula (Fmax – Fst)/Fst, which characterizes the rate of linear electron transport and is an integrated measure of photosynthesis. This indicator in the studied plants varied from of 1.86 to 2.78. The magnitude of fluorescence quenching can be placed in this order in relation to the processing of the nanocomposites and control: Nb-Saponite(Et)˃Nb-Saponite(Cl)˃ Control˃Saponite(H).

The value of Fv/F in control and experimental plants was in the range 0,75-0,77 relative units that indicates on the normal passage of photosynthesis in the control and in the research of plants.

 

References

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Published

2017-06-15

Issue

No. 3(67) (2017)

Section

Biology, biotechnology, ecology

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