RESEARCHING METABOLIC PROPERTIES OF CLINICAL STRAINS OF STAPHYLOCOCCUS WITH DIFFERENT GRADE OF RESISTANCE TO ANTIBIOTICS

Authors

  • P. S. Onischenko Oles Honchar Dnipro National University , Дніпровський національний університет ім. Олеся Гончара
  • V. G. Gavriliuk Oles Honchar Dnipro National University , Дніпровський національний університет ім. Олеся Гончара

DOI:

https://doi.org/10.31548/dopovidi2018.01.008

Keywords:

Staphylococcus, antibiotic resistance, biofilms, catabolic activity

Abstract

The ability of Staphylococcus to form biofilms was determined for monitoring the manifestation of the symptoms range of Staphylococcus clinical isolates extracted from the respiratory and digestive systems of the patients from the Diagnostic and Treatment Center (Dnipro city). As a result of cultivation experiments on the strains of Staphylococcus in vitro, a quite high film formation ability of coagulase-positive variants was revealed in 60% of cases, and only 33% of coagulase-negative cultures formed biofilms. The comparative analysis of the research results of resistance to antibiotics of Staphylococcus clinical strains showed that the film-forming variants have higher resistance levels to all studied antibiotic drugs, in contrast to isolates that didn’t form biofilms. The fact of antibiotic resistance was more often detected among coagulase-positive Staphylococcus and rarely among coagulase-negative ones. The catabolism intensity of the studied strains of Staphylococcus was determined by the total activity of dehydrogenases by the results of the recovery of triphenyltetrazolium chloride to triphenylformazan. The valuation of measurement data of the optical density of the color reaction intensity indicates that with the attainment of resistance to antibiotics, Staphylococcus catabolic metabolism ferments show less activity compared with sensitive variants and the higher the number of antibiotics which isolates of Staphylococcus have resistance to, the lower their dehydrogenase activity level. All film-forming strains proved to be more metabolically inert than Staphylococcus, which is not able to form biofilms.

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Published

2018-02-28

Issue

No. 1(71) (2018)

Section

Biology, biotechnology, ecology

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