Postnatal development of the fourth generation rats at the use of genetically modified soybeans and under influence nanoparticles of argentum

Abstract

Ukraine is the second among the European soybean producers, being also one of the world’s top ten producers. Distribution of varieties of genetically modified soybean, resistant to Roundup, increases its ability of getting it to feed. World scientists have not yet come up with an unambiguous answer to the question of the safety of transgenic crops. Therefore, the study of the long-term effects of genetically modified plants on animal organism and search for ways to reduce deviations caused by the action of their components is actuales.

In the article described results researches of influence trangenic soybeans in ration with content 35% protein on postnatal development of the fourth generation rats under the influence of nanoargentum particles.

All experiments were carried from the third generation of the Wistar line rats. All rats were divided into 4 groups: one control and three experimental. All the groups were formed by random selection of the animals with the only account of the body weight. The control group animals were fed according to the standard vivarium ration. The second group animals were fed with the food containing 35 % protein of thermotreated soybeans. The third group animals were fed using the above procedure but with the genetically engineered soybean Roundup Ready, line GTS 40-3-2: 35% protein of their diet consisted of this GM material. The fourth group of animals was formed from animals of the third group, which continued to use the experimental diet, and in the composition of their drinking water was added a solution of the preparation of Argentum nanoparticles.

Counted up the amount of living and dead rats, determined the average size of new-born, by sight fixed general physical state, shortchanged survivability, measured body mass of the rat in two months.

It has been found that the pregnancy time was unchanged in all experimental groups comparing to the value of the control group. All baby rats were delivered after 21-23 days of pregnancy. Investigated comparison of postnatal posterity development in control and experimental groups did not find out meaningful differences. The probed indexes were within the limits of physiology norms, characteristic for rats.

An average offspring number was 7.6 ± 1.3 individuals in the control group; 7.3 ± 1.6 individuals – in the second group; 7.4 ± 2.6 individuals – in the third group and 8.1 ± 2.1 individuals – in the fourth group. No deviations were found in physical development of young rats from all the groups: the auricle opening time was 3-4 days; body hair erupted after 5-6 days; teething time was 9-10 days and eyes opening time was 15-16 days. No visible mutilations were registered.

Death rate for the first five days was 6.6 % (control group), 6.9 % (second group), 8.5 % (third group) and 4.6 % (fourth group). Same rates for the next 25 days period were correspondingly 5.3 %, 5.6 %, 13.0 % and 4.8 %. It is seen that postnatal death rate for the third group animals was somewhat higher than that in the second and control group. This effect can be caused by a long term influence of phytoestrogens and other bioactive compounds coming to the organism from GM soybeans. There was a tendency to increase the number of rats in the litter and reduce their mortality in the group drinking potable water with the addition of Argentum nanoparticles concentration 0.025 μg/ml.

In animals of experimental groups, whose diet includes heat-treated soybeans, is increases body mass more actively. The total mass of rats of experimental groups at the age of two months is greater by 13-18% for the weight of animals in the control group. Apparently, this increase is due to the use of soy proteins.