Cell selection of triticum callus cultures Triticum aesticum L. on stability to agent basal bacteriosis
DOI:
https://doi.org/10.31548/dopovidi2019.04.002Keywords:
callus, Triticum aesticum L., cell selection, causative agent of basal bacteriosisAbstract
The increase in the number and severity of bacterial diseases in modern conditions of growing crops makes it necessary to use in the production of wheat varieties, which are characterized by high-quality yield for high resistance to phytopathogenic bacteria. The purpose of research. Select by the method of direct cell selection callus cultures of Triticum aesticum L., which are characterized by high growth index values and regeneration potential under the conditions of bacterial stress caused by Рseudomonas syringae pv. atrofaciens. Research methods. The material for the selection work was the seeds of winter wheat of the varieties Dark-skinned, Favorite, Metropolitan, Podolyanka. Preparation of nutrient media, introduction to culture and subculturing were carried out using traditional techniques. Results. It has been established that cell selection for resistance to the causative agent of basal bacteriosis can be carried out for the Podolyanka and Stolichnaya varieties with a sublethal concentration of 0,8 % IR P. syringae pv. atrofaciens 9400, Favoritka varieties – 0,6 %, Smuglyanka varieties – 0,4 %. The method of cell selection obtained resistant to P. syringae pv. atrofaciens, regenerative callus lines of wheat varieties Podolyanka, Stolichnaya, Favoritka and Smuglyanka.
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