Biotesting of polymeric waste excluded from solid waste waste
DOI:
https://doi.org/10.31548/dopovidi2021.06.001Keywords:
біотестування, фітотестування, Daphnia magna, Paramecium caudatum, токсикологічні дослідження, відходи полімерівAbstract
Introduction. Annually, the volume of accumulated polymers in landfills in Ukraine is growing by more than 1 million tons, but the volume of recycled polymer packaging over the past ten years has not exceeded 3%.
The goal is to establish the degree of toxicological impact on biosphere objects of polymers extracted from solid waste, to assess the safety of using household polymer waste as a secondary raw material for further processing.
The task is to establish the degree of biological and toxicological safety of polymer wastes extracted from solid waste.
Methods and techniques: toxicological - determination of water toxicity on acute Daphnia magna according to DSTU 4173: 2003 (ISO 6341: 1996, MOD) and chronic according to DSTU 4166: 2003 (ISO 10706: 2000, MOD), and Paramecium caudatum ciliates, soil microflora reactions (saprotrophic soil bacteria CFU / g) according to MR 2609-82, the assessment of phytotoxic effects on higher plants was carried out by vegetation methods according to ISO 17402-2008, ISO 17126-2005a, ISO 22030: 2005b, ISO 11269 -1:2012a.
Results. The research results show that aqueous extracts from waste polymers extracted from solid waste and their mixture do not have a pronounced toxic effect on aquatic organisms. There is no significant effect on soil bacteria. The study of the effect of polymer waste on seed germination did not reveal phytotoxic effects for any crop. A slight phytotoxic effect was observed during the study of PVC and PS waste. Wheat and mustard were the most sensitive. The level of phytotoxic effect was within acceptable limits and did not exceed 5.67%. Evaluation of phytotoxic effects on stem length showed the presence of effects from all processed products except PVC. The impact, characterized as weak, ranged from - 2.06% (PP) to - 13.27% (PS). The effect on root length was found for samples with PS waste (-7.23%), which was characterized as weak and PVC (-43.52%) - medium. Watercress and mustard were the most sensitive plants to the effects of polymer waste.
Conclusions. The studied samples of polymer waste do not show hygienically significant toxic effects on water and soil test objects, even in concentrations of 1: 1, so the impact on the above test organisms polymer waste removed from solid waste is classified as hazard class 4. According to the assessment of phytotoxic effect, polymer waste removed from solid waste is classified as hazard class 4, except for PVC - hazard class 3.
Key words: biotesting, phytotesting, Daphnia magna, Paramecium caudatum, toxicological researches, polymer wastes.
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