Investigation of the sensitivity of some microbial isolates in dogs with otitis
DOI:
https://doi.org/10.31548/dopovidi4(104).2023.009Keywords:
dogs, microbiological research, nutrient media, skin microflora, microbial isolates, otitis, staphylococci, pseudomonas, malasseziaAbstract
Introduction.The article presents the results of the study of antimicrobial sensitivity of some isolates of the skin microflora of dogs with otitis.
Methods. Methods of investigation included isolation of microbioal and fungal cultures, investigation of microbiological and biochemical features of isolates and investigation of their sensitivity to antimicrobials and antifungals.
Results. A study of the sensitivity of Malassezia pachydermatis isolates on dense nutrient media showed that posaconazole was the most effective inhibitor of Malassezia growth, as evidenced by the obtained MIC50 and MIC90 values.
Determination of sensitivity to antibiotics of representatives of the genus Pseudomonas showed that the most pronounced inhibition of the growth of microorganisms of this group was observed under the action of the following antibiotics: tobramycin, ceftazidime, marbofloxacin.
Representatives of this group of microorganisms were also highly sensitive to penicillin group - ticarcillin, fluoroquinolones norfloxacin and ofloxacin, either to aminoglycosides amikacin or gentamicin.
Discussion. Prospects of research are that further study of the sensitivity of microbial isolates of the skin to antibiotics, in particular causative agents of the cocci group, with further improvement of antibiotic therapy of animals suffering from otitis, especially in the presence of associations of causative agents of bacterial and fungal etiology.
References
Angus JC. (2004). Otic cytology in health and disease. Veterinary Clinics of North America-Small Animal Practice 34: 411-24.
Scott DW, Miller WH, Griffin CE. (2001). External ear diseases. In: Small Animal Dermatology, 6th edn. Philadelphia, PA: W.B. Saunders, 1203-35.
Paterson S, Matyskiewicz W. (2018). A study to evaluate the primary causes associated with Pseudomonas otitis in 60 dogs. J Small Anim Pract. 59(4):238-242.
Chen T, Hill PB. (2005). The biology of Malassezia organisms and their ability to induce immune responses and skin disease. Vet Dermatol. 16(1):4-26.
Borriello G, Paradiso R, Catozzi C, et al. (2020). Cerumen microbial community shifts between healthy and otitis affected dogs. PLoS ONE. 15(11):e0241447.
Tsuprun, V., Cureoglu, S., Schachern, P. A., Ferrieri, P., Briles, D. E., Paparella, M. M., & Juhn, S. K. (2008). Role of pneumococcal proteins in sensorineural hearing loss due to otitis media. Otology & Neurotology, 29(8), 1056-1060.
Buckley, L. M., McEwan, N. A., & Nuttall, T. (2013). Tris–EDTA significantly enhances antibiotic efficacy against multidrug‐resistant P seudomonas aeruginosa in vitro. Veterinary Dermatology, 24(5), 519-e122.
Glaze, M. (2013). Diseases of eyelids, claws, anal sacs, and ears. Muller and Kirk's Small animal dermatology, 741-773.
Swales N, Foster A, Barnard N. (2018). Retrospective study of the presentation, diagnosis and management of 16 cats with otitis media not due to nasopharyngeal polyp. J Feline Med Surg. 20(12):1082-1086.
Korbelik J, Singh A, Rousseau J, Weese JS. (2019). Characterization of the otic bacterial microbiota in dogs with otitis externa compared to healthy individuals. Vet Dermatol. 30(3):228-e70.
Hnilica KA. (2011). Otitis Externa Small Animal Dermatology: A Color Atlas and Therapeutic Guide. 3rd ed. St. Louis, Missouri: Elsevier Saunders; pp. 395-398.
Boyd M, Santoro D, Gram D. (2019). In vitro antimicrobial activity of topical otological antimicrobials and Tris-EDTA against resistant Staphylococcus pseudintermedius and Pseudomonas aeruginosa isolates from dogs. Vet Dermatol. 30(2):139-e40.
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