Effect of nutrient media components on regeneration ability of plant tissues culture Metasequoia glyptostroboides Hu & Cheng in vitro
DOI:
https://doi.org/10.31548/forest2019.04.073Abstract
Metasequoia glyptostroboides Hu & W. C. Cheng is a relict, endangered species included in the International Union for Conservation of Nature. Traditional methods of plant propagation often are time-consuming and inefficient. Micropropagation allows getting a big amount of healthy plants in vitro with a minimal quantity of donor material. A considerable number of biotechnological publications focus on the study of the morphogenetic potential of conifer tissues in vitro, the development of optimal protocols for their reproduction. However, most authors note the absence of a sustainable system of regeneration of woody plants in vitro, which depends on a number of factors. The purpose of the study is to determine the effect of nutrient media components on the regenerative capacity of tissues of M. glyptostroboides plants for their micropropagation and conservation in vitro. As donor plants we used a shoot parts of 10-year-old M. glyptostroboides in March 2019. All plant materials were cultured according to the conventional procedure in a light room at 24 ± 1 °C and illumination of 2 000–3 000 Lux with a 16-hour photoperiod and humidity of 70–75 %. Biotechnological and statistical methods of research were applied. The tissue, plant experiments in vitro were carried out in the research of Plant Biotechnology Laboratory of SU of NULES of Ukraine «Boiarka forest research station». A technique for introducing plant explants to in vitro conditions in spring period was developed with application of 2.5 % NaClO for 10–11 min and transfer into 1.0 % liquid of AgNO3. The significant regenerative ability of vegetal regenerates was fixed on MS nutrient medium. Active linear growth of microshoots were matched on MS medium with the addition of 0.25 mg·L-1 kinetin and 2.0 g·L-1 activated carbon. Optimal conditions for the initiation of roots formation in tissues of microshoots with a frequency of more than 60 % were created in a nutrient medium supplemented with IBA. The plant regenerates were obtained and multiplied by activating the growth of the available meristem of the explant. Further studies are aimed to develop an adaptation method of regenerating plants of M. glyptostroboides to the ex vitro and in vivo conditions for mass propagation, conservation and reproduction.
Keywords: Metasequoia glyptostroboides Hu & W. C. Cheng, plant tissue culture in vitro, explants, culture medium, regenerative ability of microshoots in vitro, microclonal propagation, microshoots in vitro.
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