Особливості отримання асептичної культури та первинний морфогенез lysimachia vulgaris l.
Abstract
Abstract. Lysimachia L. plants are a source of valuable biological components, natural antioxidants, which are actively used in the pharmaceutical industry.
The relevance has been substantiated and approaches to the microclonal reproduction of Lysimachia vulgaris L. has been developed at the stage of introduction to culture in vitro. It was established that organogenesis in the culture of L. vulgaris depends on the type of sterilizing agent and the time of exposure. It was revealed that using of sterilizing agents affects the rate and frequency of induction of primary microshoots in vitro. Combining or alternately maintaining explants of L. vulgaris in several sterilization solutions, regardless of type, is ineffective, since plant tissues are significantly damaged and incapable of morphogenesis. The active proliferation of primary micro-shoots on the nutrient medium MS supplemented with 2,5 mg∙l-1 BA and 0,2 mg∙l-1 NAA with a frequency of sprout formation of 90% was noted.
References
Belokurova, V. B., Sikura, Y. Y., & Kuchuk, M. V. (2012). Zberezhennia roslyn flory Ukrainy z riznym pryrodookhoronnym statusom v kolektsii in vitro Instytutu klitynnoi biolohii ta henetychnoi inzhenerii [Preservation of flora plants of Ukraine with different conservation status in the in vitro collection of the Institute of Cell Biology and Genetic Engineering]. Vegetational world in the Red Book of Ukraine: Implementation of the Global Strategy for Plant Conservation. Materials of the II International Scientific Conference (October 9-12, 2012, Uman, Cherkasy region), Kyiv, 223–226.
Didukh, Ia. P., Korotchenko, I. A., Fitsailo, T. V., Burda, R. I., Moisiienko, I. I., Pashkevych, N. A., Yakushenko, D. M., & Shevera, M. V. (2010). Ekoflora Ukrainy [Ecoflora of Ukraine]. Kyiv, 422.
Zholobova, O. O. (2012). Sokhranenye redkykh y yschezaiushchykh vydov rastenyi v kulture in vitro y otsenka urovnia ykh vnutryvydovoho polymorfyzma [Preservation of rare and endangered plant species in culture in vitro and assessment of their interspecies polymorphism level]. Extended abstract of candidate’s thesis. Belhorod, 23.
Konstantynov, A. V. (2014). Osobennosty reheneratsyy pobehov forsaityy evropeiskoi (Forsythia europaea Degen et Bald.) v sterylnoi kulture [Features of regeneration of European forests (Forsythia europaea Degen et Bald.) in sterile culture]. Modern Phytomorphology, 225–230.
Kushnir, H. P., & Sarnatska, V. V. (2005). Mikroklonalne rozmnozhennia roslyn. Teoriia i praktyka [Microclone propagation of plants. Theory and practice]. Kyiv, 270.
Lavrentieva, A. (2004). Vykorystannia biotekhnolohichnykh metodiv rozmnozhennia dekoratyvnykh introdutsentiv [The use of biotechnological methods of reproduction of decorative introducts]. Visnyk of Lviv University. Biological series, 137–145.
Kolomyets, T. M., Maliarovskaia, V. Y., Hvasalyia, M. V., & et al. (2014). Mikrorazmnozhenie in vitro subtropicheskih, dekorativnyh kul’tur i jendemikov Zapadnogo Kavkaza: original’nye i optimizirovannye protokoly [Micropropagation of in vitro subtropical, decorative cultures and endemics of the Western Caucasus: original and optimized protocol]. Agricultural Biology, 49–58.
Marchenko, M. M., Shelyfist, A. Ye., Cheban, L. M., Chornei, I. I., & Budzhak, V. V. (2004). Osoblyvosti vvedennia v kulturu in vitro Saussurea porcii Degen ta S. discolor (Willd.) DC. [Features of introduction into culture of in vitro Saussurea porcii Degen and S. discolor (Willd.) DC.]. Scientific Herald of Chernivtsy University, 3–9.
Radchenko, V., Matiashuk, R., Tkachenko, I., & Prokopuk, Yu. (2016). Perspektyvy praktychnoho vykorystannia predstavnykiv rodu Lysimachia L. [Prospects for the practical use of representatives of the genus Lysimachia L.]. Nitra, 377–382.
Pushkarova, N., & Belokurova, V. (2014). Osoblyvosti vvedennia v kulturu in vitro deiakykh ridkisnykh ta znykaiuchykh vydiv flory Ukrainy [Features of in vitro introduction of some rare and endangered species of Ukrainian flora]. Scientific priorities of the agrarian sphere development in the conditions of global changes: materials international. sci. pract. Internet Conf. 4-5 December 2014, Ternopil, 71–72.
Gülçin, I., Küfrevioglu, I. Ö., Oktay, M., & Büyükokuroğlu, M. E. (2004). Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J. Ethnopharmacol, 90, 205–215.
Birinci Yildirim, A., Guner, B., Pehlivan Karakas, F., & Ucar Turke, A. (2017). Evaluation of antibacterial, antitumor, antioxidant activities and fhenoloc constituents of field-grow and in vitro grown lysimachia vulgaris L. Afr. J Tradit Complement Altern Med., 14 (2), 177–187. doi:10.21010/ajtcam.v14i2.19.
Sharrock, S. (2012). Global Strategy for Plant Conservation. A guide to the GSPC: all the targets, objectives and facts. Richmond, 36.
Gupta, S., Sarma, S., Mao, A., & Seal, T. (2012). Antioxidant activity of different parts of Lysimachia laxa and Gymnocladus assamicus, a comparison using three different solvent extraction systems. Chem Pharm Res, 5, 33–40.
Gupta, S., Kaliamoorthy, S., Das, A., Mao, S., & Sarma, S. (2012). Micropropagation of Lysimachia Laxa baudo. Asian Journal of Science and Technology, 4, 12, 024–027.
Podolak, I., Elas, M., & Cieszka, K. (1998). In vitro antifungal and cytotoxic activity of triterpene saponosides and quinoid pigments from Lysimachia vulgaris L. Phytother. Res, 12, 70–73.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 473–497.
Toth, A., Riethmuller, E., Alberti, A., Vegh, K., & Kery, A. (2012). Comparative phytochemical screening of phenoloids in Lysimachia species. European Chemical Bulletin, 1, 1–2, 27–30.
Zheng, W., Xu, X.-D., Dai, H., & Chen, L.-Q. (2009). Direct regeneration of plants derived from in vitro cultured shoot tips and leaves of three Lysimachia species. Sci Hortic, 122, 138–141.
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
