Characteristics of biochemical indicators of sperm production of carp males while using naked oats in the nutrition process during pre-sprawning period

Authors

Keywords:

male carp, sexual products, sperm, total sperm activity, rectilinear translational movement, manege movement, succinate dehydrogenase, cytochrome oxidase, γ-, β- and α-globulins, albumin, prealbumin

Abstract

In the research there have been  used males of the Nyvka intra-breed type of the Ukrainian scaly carp breed, which were kept during the pre-spawning period in the pond conditions of the State Enterprise “Experimental fish farm Nyvka” within the Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine (IFNAASU). The carps were divided into 3 groups. The first group (Experiment I) was fed 100% naked oats, the second (Experiment II) - 30%, the control group of fish (Control) was fed a grain mixture. The quality of sexual products of male carp was assessed by determining the effect of adding naked oats to their diet during the pre-spawning period. Indicators of sperm motility and fertilizing ability, content of γ-, β- and α-globulins, albumin, prealbumin, and physiological characteristics of ejaculates of respiratory and regenerative capacity, activity of enzymes of respiratory chain of mitochondria (CHO and SDG) were studied. Given the increased productivity of fish sperm, in particular the increase in the number of sperm with rectilinear motion, decrease in the number of inanimate sperm and changes in the protein formula of germ cells, it is advisable to add naked oats to the main diet in the amount of 30 %.

References

Ostapiv, D. D. (2008). Indyvidualni osoblyvosti spermoproduktsii buhaiv [Individual features of bull sperm production]. Rozvedennia i henetyka tvaryn. 42. 204-210.

Yaremchuk I. M., Sharan M.M., Andrushko O.B., Korniat S.B. (2015). Zminy dynamiky rukhu spermy buhaiv-plidnykiv protiahom ekvilibratsiinoho periodu za dodavannia BAR do rozridzhuvacha [Changes in the dynamics of bulls sperm movement during the equilibrium period with addition of biologically active substances to the diluent]. Biolohiia tvaryn. 17(3). 224.

Rurangwaa E., Kime D., Ollevier F., Nash J. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture.. V. 234. P. 1 - 28.

https://doi.org/10.1016/j.aquaculture.2003.12.006

Martínez Páramo S., Gavaia P. J., Riesco M. F. (2014). Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture. 432. 389-401.

https://doi.org/10.1016/j.aquaculture.2014.04.034

Iurchak S. V., Zabytivskyi Yu. M., Deren O. V., Yaremchuk I. M. (2018). Reproduktyvni funktsii samtsiv koropa za zghodovuvannia vitaminu E ta selenu vprodovzh perednerestovoho periodu. [Reproductive functions of male carp after feeding vitamin E and selenium during the pre-spawning period] Visnyk ahrarnoi nauky. 5. 36-40.

https://doi.org/10.31073/agrovisnyk201805-06

Kazakov R.V. (1978). Opredelenie kachestva polovykh produktov samcov ryb (metodicheskie ukazaniya) [Determination of the quality of the reproductive products of male fish (guidelines)]. Leningrad: HosNYORKh. 15.

Iaremchuk I. M., Sharan M. M. (2012). Suchasni mozhlyvosti analizu yakosti spermy i rozrakhunok spermodoz [Modern possibilities of sperm quality analysis and calculation of sperm doses]. Biolohiia tvaryn. 14(1-2). 697-703.

Fauvel С., Suquet M., Cosson J. (2010). Evaluation of fish sperm quality. J. of Applied Ichthyology. 26(5) 636-643.

https://doi.org/10.1111/j.1439-0426.2010.01529.x

Skulachev V.P. (2001). Yavleniya zaprogramirovannj smerti. Mitokhondrii, kletki i organy: Rol' aktivnykh form kisloroda [Phenomena of programmed death. Mitochondria, Cells and Organs: The Role of Reactive Oxygen Species] Sorosovskij obrazovatel'nyj zhurnal. 7(6). 4-10.

Kalachniuk L.H. (2013). Aktyvnist rehuliatornykh enzymiv TsTK u klityni za rozladu travnoi systemy [ Activity of regulatory enzymes in the cell in disorders of the digestive system]. Biolohiia tvaryn. 15(3). 31-37.

Kosenko M.V., Chukhrii B.M., Kotsiumbas I.Ia. (2007). Reproduktyvna funktsiia i androlohichna dyspanseryzatsiia buhaiv [ Reproductive function and andrological examination of bulls]. Lviv, 186.

Ruiz-Pesini E., Diez C., Lapeña A.C., Pérez-Martos A., Montoya J., Alvarez E., Arenas J., López-Pérez M. J. (1998). Correlation of sperm motility with mitochondrial enzymatic activities.Clinical Chemistry. 44(8). 1616-1620.

https://doi.org/10.1093/clinchem/44.8.1616

Barbagallo F., La Vignera S., Cannarella R., Aversa A., Calogero A. E., Condorelli R. A. (2020). Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility. Journal of Clinical Medicine. 9(2). 363.

https://doi.org/10.3390/jcm9020363

Linkevich S. A., Linkevich E. Y., Zubova T. V., Sheiko E. Y., Bohdanovich D. M. (2014). Kachestvo spermy khryakov-proizvoditelej pri vvedenii novykh norm ehnergo-proteinovogo pitaniya [The quality of semen of boars-producers with the introduction of new norms of energy-protein nutrition]. Uchenye zapiski UO VHAVM. 50(2-1). 175-178.

Dudchak V. O., Sharan M. M. (2010). Vzaiemozviazok mizh aktyvnistiu suktsynatdehidrohenazy ta tsytokhromoksydazy v spermi buhaiv-plidnykiv i zaplidniuiuchoiu zdatnistiu u koriv-donoriv z indukovanoiu poliovuliatsiieiu [Relationship between succinate dehydrogenase and cytochrome oxidase activity in the semen of breeding bulls and fertility in donor cows with induced poliovulation]. Naukovyi visnyk LNUVMBT imeni S.Z. Gzhytskoho. 12. 2(44) 2. 81-85.

Söderquist Dr. L., Rodriguez‐Martinez H., Janson L. (1991). Post‐Thaw Motility, ATP Content and Cytochrome C Oxidase Activity of A. I. Bull Spermatozoa in Relation to Fertility. Journal of Veterinary Medicine Series A.. Apr; 38(3):165-174.

https://doi.org/10.1111/j.1439-0442.1991.tb00998.x

Chukhrii B. M., Klevets L. O. (1978). Do metodyky vyznachennia aktyvnosti okysliuvalnykh fermentiv u spermi buhaiv[To the method of determining the activity of oxidative enzymes in bull semen]. Rozvedennia ta shtuchne osimeninnia velykoi rohatoi khudoby. 10. 42-45.

Haal EH, Medeshy H., Veretsky L. (1982). Ehlektroforez v razdelenii biologicheskikh makromolekul [Electrophoresis in the separation of biological macromolecules]. Moscow: Mir. 446.

Borovykov V. (2001). Statistika: Iskusstvo analiza dannykh na komp'yutere [Statistics. The art of data analysis on a computer]. Sankt-Peterburh: Pyter, 656.

Downloads

Published

2021-06-29

Issue

Vol. 12 No. 2 (2021): ANIMAL SCIENCE AND FOOD TECHNOLOGY

Section

Статті

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).