QUALITATIVE INDICATORS OF RAM SPERM IN CASE OF USING REDUCED GLUTATHIONE AND BSA AS A COMPONENT OF THE EXTENDER FOR CRYOPRESERVATION

Authors

  • M. Sharan
  • K. Grymak

Abstract

The addition of 5 µМ of reduced glutathione and 15 mg/ml of BSA to LYTCGE leads to an increased activity of antioxidant enzymes (SOD and GPO) and LDH in the sperm after equilibration by 12,0, 24, 4 and 17, 5 % respectively, after freezing -thawing – by 19,7, 28, 6 and 24,1 %, as well as a decreased content of TBA-active products and a decreased activity of AST in the sperm plasma after equilibration by
22,2 and 12,1% respectively, after thawing – by 27,4 and 16,3%, which indicates a decreased damage to sperm membranes. The obtained results can serve as a basis for in -depth research on improving the extender for cryopreservation of ram sperm and
its testing in the practice of sheep breeding.

References

Деряженцев В. ?. Влияние сорбита и лактозы на криоустойчивость спермы, баранов // В. ?. Деряженцев, Т. М. Епишина // Биология воспроизведения и биотехнологические методы разведения : сб. трудов. – М. : 1992. – С. 84–86.

Ерохин А. С. Криозащитное действие новых синтетических антиоксидантов при криоконсервации спермы баранов / А. С. Ерохин, Т. М. Епишина, А. Г. Никифоров // Сельскохозяйственная биология. – 1996. – № 6. – С.1921.

Лабораторні методи досліджень у біології, тваринництві та ветеринарній медицині : довідник / [В. В. Влізло, Р. С. Федорук, І. Б. Ратич та ін.] ; за ред. В. В. Влізла. – Львів : СПОЛОМ, 2012. – 202 с.

Ali A. Studies of the effect of semi-defined diluents on the cryo-tolerance of spermatozoa and in vitro production embryos / A. Ali, B. Talib // ANNO ACCADEMICO. – 20092010. – P.138.

Ashrafi I. Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa / I. Ashrafi, H. Kohram, H. Tayefi-Nasrabadi // Spanish Journal of Agricultural Research. — 2013. – № 11 (3). – P. 695701.

Solouma, G. M. A The influence of adding glutathione on semen characteristics of sohagi rams / G. M. Solouma // Egyptian Journal of Sheep & Goat Sciences. – 2013. – Vol. 8 (2). – P. 6168.

Uysal O. Effects of Oxidized Glutathione, Bovine Serum Albumin, Cysteine and Lycopene on the Quality of Frozen-Thawed Ram Semen / O. Uysal, M. N. Bucak // ACTA VET. BRNO. – 2007. – № 76. – P. 383390.

Downloads

Issue

No. 202 (2014): Частина 1 (англ)

Section

Статті

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).