Histological changes in bone tissue during experimental mechanical damage

Authors

  • T. L. Savchuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • A. I. Mazurkevych National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. O. Maliuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • V. B. Danilov National University of Life and Environmental Sciences of Ukraine image/svg+xml

Abstract

Summarizing the results of previous studies on the nature of the optimization and stimulation of reparative osteogenesis and the potential of studying this problem, our goal was to trace the process of reparative osteogenesis in the experimental bone. Damage of the bone tissue was modeled in rabbits 3 months of age of the chinchilla breed, in the middle third of the diaphysis of the tibia. Sampling of tissues at the site of the defect, for histological studies were performed on 3, 7, 14, 21, 28 and 48 days.  The obtained histological sections from the area of injury were stained hematoxylin-eosin and examined under a microscope.

The results of studies on the histological changes in the bone tissue of rabbits during experimental mechanical damage. Established that mechanical damage to bone tissues causes a pronounced reaction from the bone tissue around the defect and newly formed bone tissue do not acquire the typical compact structure of the bone. Starting 7 days after injury, the location of the defect, there is an intense osteogenesis, and formed callus, and on 14 days the defect is completely overgrown with newly formed bone tissue.

The obtained data can be used to develop methods and means of preventing possible complications in the recovery process of damaged bone tissue, as well as for further experimental studies.

Keywords: reparative osteogenesis, callus, bone, bone marrow, histological sections, osteocytes, osteoblasts, fibroblast

References

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Published

2017-11-27

Issue

No. 273 (2017)

Section

Article

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