THE INFLUENCE OF ALLOGENEIC BONE MARROW DERIVED MESENCHYMAL STEM CELLS ON INDICATORS OF FUNCTIONAL STATE OF IMMUNE ORGANS IN MICE C57BL/6
Abstract
The studies were conducted on 2-3-months-old males of
mice weighing 20-24 g. Оur work was to study the functional state of the
organs of the immune system of C57Bl/6 mice after introduction of allogeneic
MSCs of bone marrow origin. Obtaining and cultivating of MSCs were carried
out in a sterile laminar box with compliance of conditions of asepsis and
antiseptics. C57Bl/6 mice bone marrow aspirate cultured in a CO2 incubator at
37 оC and 5 % CO2 in DMEM with 10-15 % of fetal bovine serum, 1 % of
antibiotic-antimycotic solution (Sigma-Aldrich, USA). The following groups of
animals were formed: 1 group – intact (control group); 2 group - animals, to
whom 0.5 ml of 0.9 % NaCl solution (placebo) were injected into the caudal
vein; 3 group – animals, to whom 104 of allogeneic MSCs in 0.5 ml of
phosphate buffer solution were injected into the caudal vein. The weight
index, cellularity of thymus and spleen in C57Bl/6 mice investigated after the
introduction of MSCs. The administration of allogenic mesenchymal stem cells
of the bone marrow origin affects on the central and peripheral organs of the
immune system. Administration of allogenic mesenchymal stem cells cause a
significant increase in the content of lymphoid cells in the thymus at 7 and 18
days by 72 and 39 %, respectively (p < 0.01, p < 0.05) compared to the
control. Administration of allogenic mesenchymal stem cells cause a
significant increase in the weight index of the spleen and its cellularity at the 7
and 18 days of the immune response by 26 and 17 %, respectively (p < 0.01, p
<0.05) compared to the control. the weight index of the spleen and it cellularity
a significant increase at the 7 and 18 days of the immune response by 26 and
17 %, respectively (p < 0.05) compared to the control after administration of
allogenic mesenchymal stem cells.
Keywords: mice, weight index, cellularity,
thymus, spleen,
allogenic mesenchymal stem cells
References
Aggarwal, S., Pittenger, M. G. (2005). Human mesenchymal stem cells
modulate allogeneic immune cell responses. Blood, 105, 1815–1822.
Bai, L., Lennon, D. P., Eaton, V. et al. (2009). Human bone marrow-
derived mesenchymal stem cells induce Th2-polarized immune response and
promote endogenous repair in animal models of multiple sclerosis. Glia, 57, 1192–
Bartholomew, A., Sturgeon, C., Siatskas, M. et al. (2002). Mesenchymal
stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in
vivo. Exp. Hematol., 30, 42–48.
Comoli, P., Ginevri, F., Maccario, R. et al. (2008). Human mesenchymal
stem cells inhibit antibody production induced in vitro by allostimutation. Nephrol.
Dial. Transplant., 23, 1196–1202.
Corcione, A., Benvenuto, F., Ferretti, E. et al. (2006). Human
mesenchymal stem cells modulate B-cell functions. Blood, 107, 367–372.
DelaRosa, O., Lombardo, E. (2010). Modulation of adult mesenchymal
stem cells activity by toll-like receptors: implications on therapeutic potential.
Mediators Inflamm., 2010, 865−601.
Deng, W., Han, Q., Liao, L. et al. (2005). Effects of allogeneic bone
marrow derived mesenchymal stem cells on T and B lymphocytes from BXSB mice.
DNA Cell Biol., 24, 458–463.
Di Nicola, M., Carlo-Stella, C., Magni, M. et al. (2002). Human bone
marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or
nonspecific mitogenic stimuli. Blood, 99, 3838–3843.
Djouad, F., Fritz, V., Apparailly, F. et al. (2005). Reversal of the
immunosuppressive properties of mesenchymal stem cells by tumor necrosis factor
alpha in collagen-induced arthritis. Arthr. and Rheum., 52, 1595–1603.
Spaggiari, G. M., Capobianco, A., Becchetti, S. (2006). Mesenchymal
stem cell–natural killer cell interactions: evidence that activated NK-cells are capable
of killing MSCs, whereas MSCs can inhibit IL-2–induced NK-cell proliferation. Blood,
(4), 1484–1490.
Huang, Y., Johnston, P., Zakari, A. et al. (2009). Kidney-derived stromal
cells modulate dendritic and T cell responses. J. Am. Soc. Nephrol., 20, 831–841.
Gordon, J., Manley, N. R. (2011). Mechanisms of thymus organogenesis
and morphogenesis. Development, 138 (18), 3865–3878. doi: 10.1242/dev.059998
Kladnytska, L. V., Nikulina, V. V., Garmanchuk, L. V., Mazurkevych, A. Y.,
Kovpak, V. V., Nikolaienko, T. V., Shelest, D. V., Dzhus, O. I., Skachkova, O. V.,
Stupak, Y. A., Dasyukevich, O. I. (2014). Influence allogeneic mesenchymal stem
cells on the tumour growth parameters and metastatic potential in the transplantable
carcinoma lung Lewis. Journal of Animal and Veterinary Sciences, 1 (1), 1−5.
Le Blanc, K., Rasmusson, I., Gotherstrom, C. et al. (2004). Mesenchymal
stem cells inhibit the expression of CD25 (interleukin-2 receptor) and CD38 on
phytohaemagglutinin-activated lymphocytes. Scand. J. Immunol., 60, 307–315.
Mazurkevych, A., Malyuk, M., Bezdieniezhnykh, N., Starodub, L.,
Kharkevych, Y., Jakubczak, A., Gryzinska M. (2017). Immunophenotypic
characteristics and karyotype analysis of bone marrow-derived mesenchymal stem
cells of rabbits during in vitro cultivation. Polish Journal of Veterinary Sciences, 20
(4), 687–695.
Mazurkevych, A., Kladnytska, L. V., Kovpak V. V. (2013). Features conditions
selection and cultivation mouse bone marrow adhezive fraction mononuklear cells.
Bulletin of the Taras Shevchenko National University of Kyiv, 2 (64), 41−43.
Rafei, M., Hsieh, J., Fortier, S. et al. (2008). Mesenchymal stromal cell-
derived CCL2 suppresses plasma cell immunoglobulin production via STAT3
inactivation and PAX5 induction. Blood, 112, 4991–4998.
Rasmusson, I., Le Blanc, K., Sundberg, B., Ringden, O. (2007).
Mesenchymal stem cells stimulate antibody secretion in human B cells. Scand. J.
Immunol., 65, 336–343.
Rasmusson, I., Ringden, O., Sundberg, B., Le Blanc, K. (2003). Mesenchymal
stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T
lymphocytes or natural killer cells. Transplantation, 76, 1208–1213.
Grischenko, V. А., Tomchuk, V. А. (2013). Imunomodulyuyuchi vlastyvosti
liposom na osnovi fosfolipidiv moloka pry imunodefitsitnomu stani orhanizmu tvaryn
[Immunomodulatory properties of liposomes on the basis of milk phospholipids at the
immunodeficiency state of the organism of animals]. Scientific Bulletin of the National
university of life and environmental sciences of Ukraine, 188 (4), 107−115.
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
