IMMUNOPHENOTYPIC CHARACTERISTICS AND CYTOGENETIC ANALYSIS OF MESENCHYMAL STEM CELLS OF BONE MARROW OF HORSE AT EARLY PASSAGES DURING CULTIVATION IN VITRO

Authors

  • A. Y. Mazurkevych National University of Life and Environmental Sciences of Ukraine, Kyiv
  • M. O. Maliuk National University of Life and Environmental Sciences of Ukraine, Kyiv
  • N. O. Bezdieniezhnykh R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine
  • L. F. Starodub Institute of Animal Breeding and Genetics of the National Academy of Agrarian Sciences of Ukraine

Abstract

Conducted immunocytochemical studies confirm that multipotent stem cells of horse bone marrow during cultivation in vitro in the second passage are heterogeneous. They express markers of mesenchymal, epithelial and hematopoietic cells. At the fifth passage the cell culture becomes a homogeneous immunophenotypic fraction which expresses the markers of mesenchymal origin. However, the results obtained via the cytogenetic analysis of mesenchymal stem cells of horse bone marrow in the third and fourth passages of cultivation indicate that the karyotype variability of these cells corresponds to the spontaneous level, which is typical for this species of animal.

Author Biographies

  • A. Y. Mazurkevych, National University of Life and Environmental Sciences of Ukraine, Kyiv
    Doctor of Veterinary Sciences, Professor
  • M. O. Maliuk, National University of Life and Environmental Sciences of Ukraine, Kyiv
    Candidate of Veterinary Sciences, Docent
  • N. O. Bezdieniezhnykh, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine

    Candidate of Biological Sciences, Senior Research
    Fellow

  • L. F. Starodub, Institute of Animal Breeding and Genetics of the National Academy of Agrarian Sciences of Ukraine
    Candidate of Agricultural Sciences, Senior Research Fellow