THE EFFECT OF ALLOGENEIC MESENCHYMAL STEM CELL TRANSPLANTATION ON THE ACTIVITY OF MITOCHONDRIAL SUCCINATE DEHYDROGENASE IN THE LIVER OF RECIPIENT ANIMALS
DOI:
https://doi.org/10.31548/ujvs2019.03.001Abstract
The studies were conducted on 2-3-months-old males of C57BL/6 mice weighing 20-24 g. Оbtaining of allogenic bone marrow and adipose derived MSCs were carried out in a sterile laminar box. Cells were cultivated in a CO2-incubator at 37 оC and 5 % CO2 in DMEM with 10-15 % of fetal bovine serum, 1% of antibiotic-antimycotic solution (Sigma-Aldrich, USA). The following groups of animals were formed: 1 group – intact (control) animals; 2 group – animals, to whom 0.5 ml of 0.89% NaCl solution (placebo) were injected into the caudal vein; 3 group – animals, to whom 104 of allogenic AD MSCs in 0.5 ml of phosphate buffer solution were injected into the caudal vein, 4 group – animals, to whom 104 of allogenic BM MSCs in 0.5 ml of phosphate buffer solution were injected into the caudal vein. On 12th day after transplantation of MSCs activity of succinate dehydrogenase in animals were determined.
The determination of the activity of the enzyme was carried out in accordance with the method, the principle of which is the restoration of potassium ferricyanide (K3[Fe(CN)6]) to potassium ferrocyanide (K4[Fe(CN)6]) by succinate with involvement of succinate dehydrogenase. Activity was determined by the volume of recovered ferricyanide. Statistical processing of the results was performed with using of "Origin 6.1" software and Student's t-criterion. All data are presented in the form of arithmetic average and standard deviations.
It was found that at the 12 day of the study, the activity of the succinate dehydrogenase in the third experimental group was 57.7 ± 1.6 mmol / l K3[Fe (CN)6] / mg * min (p <0.001), which is significantly higher than in the animals of the first and second group (45.9 ± 0.7 and 43.3 ± 1.2 mmol / l K3[Fe (CN)6] / mg * min, respectively). The activity of the enzyme in animal of the fourth experimental groups was also reliable higher compared to the first two groups and was 53.3 ± 1.4 mmol/l K3[Fe(CN)6] / mg * min (p < 0.01).
It should be noted that mitochondrial succinate dehydrogenase activity in the liver of recipient animals after transplantation of MSCs from adipose tissue is significantly higher than after transplantation of MSCs, obtained from bone marrow (p < 0.05). Thus, reliable raising of mitochondrial succinate dehydrogenase activity in the liver of recipient animals after transplantation of allogenic mesenchymal stem cells from adipose tissue and bone marrow has been determined.
Keywords: mesenchymal stem cells, mitochondria, succinate dehydrogenase, mice, bone marrow, fatty tissue.
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