Influence of vitamins E and C on the quantity and functional activity of T- and B-lymphocytes of blood-chicken broilers
DOI:
https://doi.org/10.31548/ujvs2020.01.007Abstract
Abstract. Separate indices of T- and B-cell immunity of broiler chickens were investigated during the period of their growth with additional introduction of tocopherol acetate and ascorbic acid into their diet. The studies were conducted in one of the farms in the Lviv region on four groups of 100 broiler chickens each, ranging from 1- to 41 days of age. Chickens in the control group received a standard diet, the first experimental diet with the addition of tocopherol acetate, the second experimental - ascorbic acid, and the third - a complex of these vitamins. In whole blood, the relative number of T and B lymphocytes and their individual populations were determined in the reaction of spontaneous rosette formation with ram erythrocytes, differentiated calculation of rosette lymphocytes with different degree of functional activity was performed.
For research, blood was collected from broiler chickens after slaughter at different ages: 27-, 34-, and 41-day-olds. During the work, the bioethical requirements of the animals were complied with according to the current legislation.
Studies have shown that the additional introduction of chickens to their diet tocopherol acetate and ascorbic acid has a stimulating effect on the number of T and B lymphocytes and the functional activity of T and B cell units of immunity. This is evidenced by the higher number of (-lymphocytes (common, active, theophylline-resistant) and Β-lymphocytes in the blood of broiler chickens, which were supplemented with vitamins E and C. Τ- and Β-lymphocyte apparatus - an increase in the number of cells with low and medium degrees of aperture and a decrease in the inactive function of Τ- and Β-lymphocytes in the blood. These changes were more pronounced in the blood of chickens at 27 days of age due to the action of vitamin C, as well as the complex of tocopherol acetate and ascorbic acid.
Therefore, on the basis of the conducted researches it is possible to confirm a regulatory role of vitamins E and C in formation of a cellular link of specific immune response of chickens-broilers. The action of the investigated vitamins, and especially of ascorbic acid, revealed an increase in the number and increase in the functional activity of T- and B-lymphocytes of the blood. These changes are probably due to the antioxidant action of vitamins in providing metabolic homeostasis of membranes and the receptor apparatus of immunocompetent cells.
References
Vlizlo, V. V., Kurtiak, B. M., Vudmaska, I. V., Vishchur, O. I., Petruk, A. P. (2015). Zhyrorozchynni vitaminy u veterynarnii medytsyni ta tvarynnytstvi [Fat-soluble vitamins in veterinary medicine and animal husbandry] 2-he vyd., dop. Lviv: Spolom, 226-227. (in Ukrainian)
Voronin, E. S., Petrov, A. M., Seryh, M. M., Dervishov, D. A. (2002). Immunologija [Immunology]. Pod red. E.S. Voronina. M.: Kolos-Press, 408 (in Russian)
Ionov, I. A. (1997). Vitaminy E i S kak komponenty antioksidantnoj sistemy jembrionov ptic i mlekopitajushhih [Vitamins E and C as components of the antioxidant system of bird and mammalian embryos]. Ukr. biohim. zhurn., 69(5-6): 3-11. (in Russian)
Lemesheva, M. M. (2003). Hodivlia silskohospodarskoi ptytsi. Sumy [Feeding farm animals and poultry], 152. (in Ukrainian)
Maslianko, R. P. (1999). Osnovy imunolohii [Fundamentals of immunology]. Lviv: Vertykal, 427. (in Ukrainian)
Mudrak, D. I., Vishchur, O. I. (2011). Kilkist T- i V-limfotsytiv ta yikh funktsionalna aktyvnist u krovi husei za riznoho rivnia vitaminiv E i S u ratsioni [The number of T and B lymphocytes and their functional activist in the blood of geese at different levels of vitamins E and C in the diet]. Biolohiia tvaryn, 13(1-2):427-430. (in Ukrainian)
Mudrak, D. I., Vishchur, O. I., Broda, N. A. et al. (2012). State T- and B-cell immunity in parts of turkeys and geese for various levels of vitamin E the diet [Statʹy T- end B-tselʹ immuniti inpart·s of turke and geese for varius levels of vitamin E tkhe diyet]. The Animal Biology, 14(1-2):535-539. (in Ukrainian).
Osobenosti immunnoj sistemy u pticy [internet resurs]. Rezhym dostupu: www.vetom.ru/content/view/515/427/1/13. (in Russian)
Rojt, A., Brostoff, Dzh., Miel, D. (2000). Immunologija [Immunology]. Per. s angl. M.: Mir, 592. (in Russian)
Silonov, S. B., Donchenko, H. V., Morozova, R. P. (2008). Osoblyvosti obminu leikotryieniv v orhanizmi shchuriv za umov E-hipovitaminozu [Features of leicotriene metabolism in rats under conditions of E-hypovitaminosis]. Ukrainskyi biokhimichnyi zhurnal, 80(1):26-32. (in Ukrainian)
Fisinin, V., Suraj, P. (2013). Immunitet v sovremennom zhivotnovodstve i pticevodstve: ot teorii k praktike immunomoduljacii. Pticevodstvo [Immunity in modern animal husbandry and poultry farming: here the theory to the practice of immunomodulation. Poultry farming], 5:4-10. (in Russian)
Haitov, R. M., Ignat'eva, G. A., Sidorovich, I. G. (2000). Immunologija [[Immunology]. M.: Medicina, 432. (in Russian)
Bartha, J. L., Comino-Delgado, R. (2000). Lymphocyte subpopulation after normal prig nancy and spontaneous abortion in primigravidas. J. Repr. Med. Obstet. Gynec., 45:567-572.
Colombo, M. L. (2010). An update on vitamin E, tocopherol and tocotrienol-perspectives. Molecules, 15(4): 2103-2113.
https://doi.org/10.3390/molecules15042103
Gisela, F. (1997). Err Immune system function and development in broilers. Center of Excellence for Poultry Science University of Arkansas. Fayetteville, 102-123.
King, A., Burrows, T., Verna, S. (1998). Human uterine lymphocytes. Human Repr. Update., 4:480-485.
https://doi.org/10.1093/humupd/4.5.480
Maxrygimas, G., Plachoural, V., Higneras, M. et al. (1993). Maternal peripheral blood lymphocyte subpopulations in normal and pathological hrtgnancies. Fetal Diagn. Ther., 178(9):371-378.
https://doi.org/10.1159/000264068
Medina, R. J., Smithson, G., Kincade, R. W. (1993). Supression of B lymphocyte during normal pregnancy. J. Exp. Med., 178: 1507-1515. https://doi.org/10.1084/jem.178.5.1507
Thoman, M. L. (1995). The pattern of T-lymphocyte differentiation is altered during thumic involution. Mech. Ageing Dev., 82:155-170. https://doi.org/10.1016/0047-6374(95)01597-S
Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4):536-541.
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