Interconnectionbetweenexpression of Fc-γ-receptor proteins with the activity of individual enzymes in the plasmalemma enterocytes of the jejunum intestine of the bovine fetus
DOI:
https://doi.org/10.31548/ujvs2020.01.008Abstract
Abstract. Knowledge of the patterns of development of digestive organs in productive animals during prenatal ontogeny is a biological prerequisite to ensure an adequate level of their vitality at birth. The article deals with the interconnection of expression of Fc-γ receptor proteins with the activity of individual enzymes in plasmalemma of jejunum enterocytes of cattle fetus. Immunoblotting revealed Fc-γ receptor proteins of the apical and basolateral membranes of the jejunum enterocytes with a molecular weight of 120 kDa, 87 kDa, 72 kDa, and 43 kDa. In the apical membrane of enterocytes, the activity of γ-glutamyltransferase, lactase, Na+, K+ -ATPase, Ca2+, Mg2+ -ATPase and Mg-ATPase is directly related to the content of Fc-γ-receptor proteins of the enterocyte membrane with a molecular weight of 43 kDa(r = 0,77–0.92; P ≤ 0.05–0.01), and the activity of Mg2+ -ATPase and lactase inversely correlates with the content of Fc-γ-receptor proteins of the apical membrane of enterocytes with a molecular weight of 87 kDa (r = 0.75–0 , 76; P ≤ 0.05). In the basolateral membrane of enterocytes, alkaline phosphatase activity is directly related to the content of Fc-γ-receptor proteins with a molecular weight of 87 kDa (r = 0.99; P ≤ 0.001) and inversely related to the content of proteins with a molecular weight of 43 kDa (r = -0.79; P ≤ 0.05). The activity of γ-glutamyltransferase is directly related to the content of Fc-γ-receptor proteins with a molecular weight of 87 kDa and 72 kDa (r = 0.73–0.79; Р ≤ 0.05) and inversely related to the protein content of the molecular weight 120 kDa (r = -0.77; P ≤ 0.05). Lactase activity is directly related to the content of Fc-γ-receptor proteins with a molecular weight of 72 kDa (r = 0.82; P ≤ 0.05) and inversely related to the content of proteins with a molecular weight of 120 kDa (r = -0,80; P ≤ 0.05). The results of the research have both fundamental and applied value.The peculiarities of interconnection expression of Fc-γ receptor proteins with the activity of individual enzymes in the apical and basolateral membranes of enterocytes open the perspectivefor further research of the physiological and biochemical aspects of membrane digestion in productive animals in prenatal and early postnatal in early postnatal ontogeny as a basis for the disclosure of pathogenesis of gastrointestinal disorders in animals
Keywords: Fc-γ-receptors, jejunum intestine, plasmalemma, enterocytes, ATP-ase, hydrolasesReferences
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