Influence of phospholipid-containing additives on the functional condition of organs and systems of mice
DOI:
https://doi.org/10.31548/ujvs2020.03.002Keywords:
milk phospholipids, BAA "FLP-MD", laboratory mice of the CBA line, clinical, hematological and microscopic examinations, functional state of the organismAbstract
Abstract. Numerous studies in experiments on laboratory animals and in clinical practice have confirmed the positive effect of essential phospholipids on the structural organization of cell membranes, intracellular metabolism, mitochondrial function, antioxidant processes, which explains the active use of phospholipid-containing drugs in hepato- and enteropathologies, cardiovascular diseases, etc. Raw material of animal origin, in particular milk, which is a safe source of biologically active substances, has a structural similarity with phospholipids of mammalian tissues. Based on them, a biologically active additive (BAA) "FLP-MD" was created, which is characterized by membraneotropic properties and reparative effect. The aim of work was to investigate the biological effect of the liposomal form of BAA "FLP-MD" based on milk phospholipids on the body of clinically healthy laboratory mice.
Determination of the toxicity of phospholipid-containing BAA "FLP-MD" was performed on mice of the CBA line. The duration of the experiment was 16 days. The results of the studies indicate a probable increase in body weight of mice of the CBA line after the 10th day of intragastric administration of 1% solution of liposomal form of BAA "FLP-MD".
As a result of the study of biochemical parameters of plasma / native blood, the absence of toxic effects of liposomal form of BAA "FLP-MD" on the functional state of the body of mice was set. Morphological parameters of peripheral blood in these animals did not change significantly. According to the results of microscopic studies, no negative effect of the liposomal form of BAA "FLP-MD" on the structure microstructure of the mucous membrane of the gastrointestinal tract was found.
Therefore, the components of BAA "FLP-MD" do not adversely affect the functional state of organs and systems of laboratory mice and do not show a toxic effect, which allows it to be recommended for use in veterinary medicine as a means of rehabilitation therapy.
Keywords: milk phospholipids, BAA "FLP-MD", laboratory mice of the CBA line, clinical, hematological and microscopic examinations, functional state of the organism
References
Bothra, A., Arumugam, P., Panchal, V., Menon, D., Srivastava, S., Shankaran, D., Nandy, A., Jaisinghani, N., Singh, A., Gokhale, R. S., Gandotra S., & Rao V. (2018). Phospholipid homeostasis, membrane tenacity and survival of Mtb in lipid rich conditions is determined by MmpL11 function. Scientific Reports, 8(8317):1-14.
https://doi.org/10.1038/s41598-018-26710-z
Calitz, C., Hamman, J. H., Fey, S. J., Wrzesinski, K., & Gouws, C. (2018). Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective. Toxicology Mechanisms and Methods, 28(5):369-385.
https://doi.org/10.1080/15376516.2017.1422580
Colombo, S., Melo, T., Martinez-Lopez, M., Carrasco, M. J., Domingues, P., Pérez-Sala, D., & Domingues, R. (2018). P-150 - Phospholipidome of endothelial cells shows a different adaptation response upon oxidative, glycative or carbonyl stress. Free Radical Biology and Medicine, 120(1):S90. https://doi.org/10.1016/j.freeradbiomed.2018.04.297
Gariani, K., Ryu, D., Menzies, K. J., Yi, H.-S., Stein, S., Zhang, H., Perino, A., Lemos, V., Katsyuba, E., Jha, P., Vijgen, S., Rubbia-Brandt, L., Kim, Y. K., Kim, J. T., Kim, K. S., Shong, M., Schoonjans, K., & Auwerx1, J. (2017). Inhibiting poly ADP-ribosylation increases fatty acid oxidation and protects against fatty liver disease. Journal of Hepatology, 66(1):132-141. https://doi.org/10.1016/j.jhep.2016.08.024
Gryshchenko, V. A. (2019). Blood and acid composition of blood and biles in calves at enteropatology and application of milk phospholipides. Ukrainian Journal of Veterinary Sciences, 10(4): 36-42.
https://doi.org/10.31548/ujvs2019.04.005
Gryshchenko V., Danchenko O., & Musiychuk V. (2019) Modification of Modeling Method of Toxic Dystrophy of Liver in Rats. In: Nadykto V. (eds) Modern Development Paths of Agricultural Production. Springer, Cham.
https://doi.org/10.1007/978-3-030-14918-5_67
Gryshchenko V. А., Sуsolyatin S. V., & Gulevata J. V. (2018). Phospholipid composition of blood plasma and internal organs of rats with diclofenac-induced hepatitis. Ukrainian Journal of Ecology, 8(3): 211-215.
Gryshchenko, V., Sуsoliatin, S., & Midyk, S. (2019). Fatty acids of lipids of blood serum and liver of rats with tetracyclin-induced hepatosis and at correction. Regulatory Mechanisms in Biosystems, 10(4):520-525.
https://doi.org/10.15421/021976
Gula, N. M., & Margitich, V. M. (2009). Zhyrni kysloty ta yikh pokhidni pry patolohichnykh stanakh [Fatty acids and their derivatives in pathologic starts]. Naukova dumka, Kyiv (in Ukrainian).
Melnychuk, D. O., Gryshchenko, V. A., & Lytvynenko, O. N.: Veterynarna biologichno aktyvna dobavka liposomal'noi' formy ta sposib reparatyvnoi' terapii' v gepatologii' [Veterinary biologically active additive of liposomal form and method of reparative therapy in hepatology]. Patent UA, 86516 (2009). (in Ukrainian).
Sang-Chul, K. & Xuemin, W. (2020). Phosphatidic acid: an emerging versatile class of cellular mediators . Essays in Biochemistry. EBC20190089. https://doi.org/10.1042/EBC20190089
Solís-Calero, C., Ortega-Castro, J., Frau, J. & Muñoz, F (2015). Nonenzymatic reactions above phospholipid surfaces of biological membranes: reactivity of phospholipids and their oxidation derivatives. Oxidative Medicine and Cellular Longevity, 319505:22.
https://doi.org/10.1155/2015/319505
Starr, M. L., Sparks, R. P., Arango, A. S., Hurst, L. R., Zhao, Z., Lihan, M., Jenkins, J. L. Tajkhorshid, E., & Fratti, R. A. (2019). Phosphatidic acid induces conformational changes in Sec18protomers that prevent SNARE priming. Journal of Biological Chemistry, 294:3100-3116.
https://doi.org/10.1074/jbc.RA118.006552
Tomchuk V., Gryshchenko, V., Vlizlo, V., & Enciu V. (2019). Сorrective effect of milk phospholipids in pathological conditions. Iaşi Editura Ion Ionescu de la Brad.
Udut, V. V., Vengerovsky, A. I., & Dygai, A. M. (2013). Effects of phospholipid hepatoprotectors on apoptosis during experimental liver pathology induced by isoniazid and paracetamol. Bulletin of Experimental Biology and Medicine, 154(5):614-617.
https://doi.org/10.1007/s10517-013-2012-9
Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni [Laboratory methods of investigation in biology, stock-breeding and veterinary]. Spolom, Lviv (in Ukrainian).
Yang, K. J., Son, J., Jung, S. Y., Yi, G., Yoo, J., Kim, D. K., & Koo, H. (2018). Optimized phospholipid-based nanoparticles for inner ear drug delivery and therapy. Biomaterials, 171(7):133-143.
https://doi.org/10.1016/j.biomaterials.2018.04.038
Zhang, X., Yang, L., Liu, Y., Song, Z., Zhao, J., Chen, D., Yu, H., Li, R., Wang, Y., Yang, K., Chen, Y., Xia, M., & Zhang, L. W. (2018). Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis. Toxicology and Applied Pharmacology, 348:54-66.
Downloads
Published
Issue
Section
License
Relationship between right holders and users shall be governed by the terms of the license Creative Commons Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
