Вплив фосфоліпідвмісних препаратів на рівень імуноглобуліна G в сироватці крові телят у період формування колострального імунітету

Автор(и)

  • S. I. Golopura Національний університет біоресурсів і природокористування України image/svg+xml
  • M. I. Tsvilikhovsky Національний університет біоресурсів і природокористування України image/svg+xml
  • B. V. Popadiuk Національний університет біоресурсів і природокористування України image/svg+xml

DOI:

https://doi.org/10.31548/ujvs2020.01.001

Анотація

Abstract. The results of the application of the medication “Membranostabil” and native phospholipid bilayer liposomes based on soybean lecithin developed by this research group for correction of serum immunoglobulin G (IgG) content in newborn calves during colostral immunity formation are presented. The indices of IgG content in blood serum of newborn calves have been investigated in dynamics - from the birth till the age of 11 days. Studies were performed on newborn calves of three groups (control, and two experimental ones) of the Ukrainian black-and-white dairy breed. The level of IgG was investigated by electrophoresis in polyacrylamide gel Quantitative estimation of protein fractions was performed by scanning the electrophoregram, with their subsequent graphical reconstruction and calculation by relative units or area using a computer program. It is established that “Membranostabil” medication and native liposomes from phospholipid bilayer based on soybean lecithin activate transport of immunoglobulins in small intestine and promote a significant increase of content of serum IgG compared to calves of the control group. The content of IgG in the serum of newborns calves of both experimental groups at 6 hours of age significantly increased and remained higher throughout the duration of the experiment, except for the calves of the first experimental group at the age of 7 days, compared with calves in the control group. The dynamics with comparative analysis of serum IgG content between calves of individual groups is shown. The increase of content of serum IgG in newborn calves is one of factors preventing early immunodeficiency, sepsis, development of digestive disorders, and other diseases of young animals.

Keywords: colostral immunity, colostrum, immunoglobulin G (IgG), newborn calves, Membranostabil

Посилання

Borghesi, J., Mario, L. C., Rodrigues, M. N., Favaron, P. O., Miglino, M. A. (2014). Immunoglobulin Transport during Gestation in Domestic Animals and Humans - A Review. Open Journal of Animal Sciences, 4:323-336. https://www.scirp.org/html/15-1400256_51036.htm

https://doi.org/10.4236/ojas.2014.45041

Palmeira, P., Quinello, C., Silveira-Lessa, A. L., Zago, C. A., Carneiro-Sampaio, A. (2012). IgG Placental Transfer in Healthy and Pathologica Pregnancies. Clinical and Developmental Immunology, 1-13. doi:10.1155/2012/985646

https://doi.org/10.1155/2012/985646

Jim Quigley. (2007). Passive Immunity in Newborn Calves WCDS Advances in Dairy Technology, 19: 247-265.

Silper, B. F., Coelho, S. G., Madeira, M. F., Ruas, J. M., Lana, A. Q., Reis, R. B., Saturnino, H. M. (2012). Avaliação da qualidade do colostro e transferência de imunidade. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 64:281-285.

https://doi.org/10.1590/S0102-09352012000200005

Davis, C. L.; Dracley, J. K. (1998). The development, nutrition, and management of the young calf. 3.ed. Iowa, USA, 339.

Mallery, D. L., McEwan, W. A., Bidgood, S. R., Towers, G. J., Johnson, C. M., James, L. C. (2010). Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21). Proceedings Of The National Academy Of Sciences Of The United States Of America,107 (46):19985-19990.

https://doi.org/10.1073/pnas.1014074107

Dzhanabekova, H. K., Aldanazarov, S. S., Zhumashev, Zh. Zh., Dzhanabekov, K., Ysemberhenova, S. K., Dzhunusova, R. Zh., Zhyilkyishyibaeva, M. M. (2012). Izuchenie aminokislotnogo sostava immunoglobulina G1 krupnogo rogatogo skota [The study of the amino acid composition of cattle immunoglobulin G1]. Issledovaniya, rezultatyi. https://articlekz.com/article/12830 (in Russian)

Ofitserov, V. I. (2005). Podklassyi immunoglobulina G: vozmozhnosti ispolzovaniya v diagnosticheskoy praktike [Subclasses of immunoglobulin G: possibilities for use in diagnostic practice]. Koltsovo, 19. (in Russian)

Atkinson, D. E., Boyd, R. H., Sibley, C. P. (2006). In vitro methods for studying human placental amino acid transport placental villous. Placental Transfer. Elsevier, Manchester, 2787-2846.

https://doi.org/10.1016/B978-012515400-0/50057-9

Pires, J. B. (2009). Evaluation of Passive Transfer of Immunity in Newborn Calves Derived from Dystocia Obtained by Caesarian Section. Dissertation, Universidade Rural de Pernambuco, Recife.

Wang, C. W., Yang, W. D., Yang, W. Y., Lv, W. F., Yang, L. Y. (2015). Effect of acanthopanax synbiotics on serum TAOC, IgG and IgA of lactation calves. Chin J Vet Med, 51(2):48-50.

Tsvilikhovsky, M. I., Maryniuk, M. O., Holopura, S. I., Avdieieva, L. Yu., Nemova, T. V., Yakymchuk, O. M., Zhukotskyi, E. K. (2014). Patent Ukrainy 92841 Kyiv: Derzhavne patentne vidomstvo Ukrainy. (іn Ukrainian)

Schägger, H., Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry, 166 (2):368-379.

https://doi.org/10.1016/0003-2697(87)90587-2

Brandon, M. R., Watson, D. L., Lascelles, A. K. (1971). The mechanism of transfer if immunoglobulin into mammary secretion of cows. Aust. J. Esp. Biol. Med. Sci, 49:613-623.

https://doi.org/10.1038/icb.1971.67

Ježek, J., Malovrh, T., Klinkon, M. (2012). Serum immunoglobulin (IgG, IgM, IgA) concentration in cows and their calves. Acta agriculturae Slovenica, 3:295-298.

Holopura, S. I., Tsvilikhovsky, M. I., Popadiuk, B. V. (2019). Influence of medication "Membranostabil" on expression of immunoreceptor proteins in small intestine of ruminants during the period of formation of colostral immunity. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21 (96):147-152. DOI: https://doi.org/10.32718/nvlvet9626

https://doi.org/10.32718/nvlvet9626

Golopura, S. I., Tsvilikhovsky, M..I., Popadiuk, B.V. (2019). Influence of membrane-repairing medications on the expression of proteins of plasmolemma of enterocytes during the formation of colostral immunity. Scientifics reports of NULES of Ukraine, 6 (82).

https://doi.org/10.31548/dopovidi2019.06.019

<http://journals.nubip.edu.ua/index.php/Dopovidi/article/view/13464>

Nonnecke, B. J., Waters, W. R., Goff, J. P., Foote, M. R. (2012). Adaptive immunity in the colostrum-deprived calf: Response to early vaccination with Mycobacterium bovis strain bacilli Calmette Guerin and ovalbumin. Journal of Dairy Science, 95:221-239.

https://doi.org/10.3168/jds.2011-4712

Singh, A. K., Pandita, S., Vaidya, M. M., Chandra, G., Kushwaha, R. (2011). Colostral immunoglobulins and neonatal immunity in bovine. Wayamba Journal of Animal Science, 578:78-84.

Aleksandrova, E. A., Gaydasheva, E. V., Burnevich, E. Z. (2010). Hronicheskaya intoksikatsiya vitaminom А kak prichina formirovaniya tsirroza pecheni. [Chronic intoxication with vitamin A as a cause of liver cirrhosis] Farmateka 10: 37-41. (in Russian).

Kurtyak, B. M., Yanovich, V. G. (2004). ZhirorozchinnI vitamini u veterinarniy meditsini i tvarinnitstvi [Fat-soluble vitamins in veterinary medicine and animal husbandry] Lviv: Triada plyus, 426. (in Ukranian).

Butler, J. E. (1998). Immun0oglobulin diversity, B-cell end antibody repertoire development in large farm animals. Rev. sci. tech. Off. int. Epiz., 17 (1): 43-70.

https://doi.org/10.20506/rst.17.1.1096

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Опубліковано

2020-03-19

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