The effectiveness of amniotic membrane depending on the cause of corneal damage in dogs

Автор(и)

  • O. V. Shupyk Національний університет біоресурсів і природокористування України image/svg+xml
  • A. Y. Mazurkevych Національний університет біоресурсів і природокористування України image/svg+xml
  • R. R. Bokotko Національний університет біоресурсів і природокористування України image/svg+xml

DOI:

https://doi.org/10.31548/ujvs2020.04.006

Ключові слова:

Key words, clinical cases of keratoconjunctivitis, dogs of different breeds, mesenchymal stem cells (MSCs), amniotic membrane transplantation (TAM), corneal condition

Анотація

Abstract.  The results of studies on clinical cases of keratitis in dogs of different etiology, using measuring instruments and visual methods, after transplantation, amniotic membrane, which is the inner of the three amniotic membranes, transparent, avascular, developing from the fetal ectoderm and consisting of epithelial layer,  located on the main membrane and connective tissue stroma.  These results make it possible to analyze and further study the effect of allogeneic mesenchymal stem cells in amniotic membrane transplantation in various defects of the eye in general, as well as corneas with ulcers of various etiologies.

 Eye injuries and chemical burns are ophthalmic emergencies that require immediate diagnosis and treatment, as they can lead to deterioration and loss of vision. Clinical manifestations of such burns are caused by exacerbation of the innate immune response due to infiltration of inflammatory cells and activation of stromal fibroblasts. New treatments are emerging that address restorative mechanisms that improve the surface of the eye after injury; for example, stem cell transplantation (SC) has been successfully reported for this purpose. (Stem Cells Translational Medicine 2018;7:906–917).

The main task of the study is to prove the effectiveness of the amniotic membrane to indicate: activation of proliferation, stopping tissue apoptosis, reducing the activity of the inflammatory process, accelerating epithelialization and normalization of the corneal stroma, which leads to normal restoration of transparency without vascularization and turbidity. Also factors due to the effect of specific placental properties that regulate metabolism in tissues and embryonic leaves of the fetus, which have the ability to regenerate tissues and to activate their own stem cells, regardless of the cause of damage.

 

Посилання

Gololobov V.G., Gaivorovsky I.V., Deev R.V., Rudko A.S., Ellinidi V.N., Anikeeva N.V., Sukhinin M.V. Reparative regeneration of multilayered corneal epithelium: biotechnological potential. Cell transplantology and tissue engineering. 2008; 3 (4): 55-9

Kadyshev V.V., Kadysheva L.V. Use of corneal regeneration stimulants for traumatic injuries. Clinical ophthalmology. 2011; 12 (4): 153-5

Mylyudyn Е.С. Experimental model of regional corneal epithelial stem cell insufficiency. Vestnik SamGU. Natural science series. 2006; 9 (49): 219-26

Puchkovskaya N.A., Yakimenko S.A., Nepomnyashchaya V.M. Eye burns. M .: Medicine, 2001: 272

Radchenko A.V., Shevlyuk N.N., Kirillichev A.I. Morphofunctional characteristics of reparative histogenesis in the cornea of rabbits with burn damage to the limbal region. Bulletin of Orenburg State University. 2014; 13 (174): 85-7

Radchenko A.V., Shevlyuk N.N., Kirillichev A.I. Morphofunctional characteristics of reparative histogenesis of rabbit corneal tissues after thermal damage to the limbal region. Morphology. 2013; 144 (5): 108-9

Rapuano K. J. Cornea: lane. with English M .: GEOTAR-Media, 2010: 320

Chernysh VF, Boyko EV Eye burns are a condition and new approaches. SPb .: VMedA, 2008: 135

Churashov S.V., Chernysh V.F., Rudko A.S., Zlobin I.A. Features of healing of severe alkaline burns only of the cornea, only of the limbal zone, as well as their combination in the experiment. Ophthalmology. 2012; 4-2 (59): 214-7

Daniels JT, Dart JK, Tuft SJ, Khaw PT. Corneal stem cells in review. Wound Repair and Regeneration. 2001 Nov; 9 (6): 483-94.

https://doi.org/10.1046/j.1524-475x.2001.00483.x

Daya SM, Watson A, Sharpe JR, Giledi O, RowemA, Martin R, et al. Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction. Ophthalmology. 2005 Mar; 112 (3): 470-7.

https://doi.org/10.1016/j.ophtha.2004.09.023

Gupta N, Kalaivani M, Tandon R. Comparison of prognostic value of Roper Hall and Dua classification systems in acute ocular burns. British Journal of Ophthalmology. 2010 Aug 5 (2): 94.

https://doi.org/10.1136/bjo.2009.173724

Tseng SCG, Liang L, Sheha H. Liand limbal stem cell transplantation: new progresses and challenges. Eye. 2008; 1 (8): 34-8.

Wagoner MD. Chemical injuries of the eye: Current concepts in pathophysiology and therapy.

Survey of Ophthalmology. 1997 Jan; 41 (4): 275- 313

https://doi.org/10.1016/S0039-6257(96)00007-0

Завантаження

Опубліковано

2020-12-02

Номер

Розділ

Статті