Improvement of treatment and prophylaxic measures for a mutual course of leptospyrosis and babezidios in the horses

Abstract

The article presents the hematological and serological results of research of blood of horses for the joint course of leptospirosis with babesijidazami and found effective methods of treatment. The material for research was stabilized blood and serum from the horses of patients with leptospirosis and babesiosis. From animals with a compatible course of diseases on the principle of analogues were formed three groups of 5 head in each, in the fourth - the control group consisted of 5 clinically healthy horses, seronegative for leptospirosis. In all three experimental groups of animals, before and after treatment (at intervals of 14 days) blood was collected and hematological and biochemical blood tests were performed. Also, from the peripheral blood, smears were prepared, stained them according to the method of Romanovsky and examined under a microscope for presence in beryozoites in erythrocytes. It has been established that in patients with horses there is a significant (P < 0.001-0.05) decrease in the content of hematocrit, hemoglobin, total protein, and the number of red blood cells. However, in patients with horses there is a significant (P < 0.01) increase in the content of immunoglobulins and the number of leukocytes. Animals of the first group received treatment in the form of intravenous administration of beryllin (azidine) in a dose of 3.5 mg / kg body weight together with 5 % glucose solution in a dose of 1 ml / kg body weight and 20 % solution of sodium caffeine sodium benzoate at a rate of 1 , 5 ml / 100 kg body weight of the animal. Animals of the second group used intravenous Diminacel plus (3.5 mg of diazeturatum diaminase / kg body weight or 5 ml of 7 % solution / 100 kg of body weight) with the preparation of CGEP (consisting of sulfanilamide nonsulfazole, glucose, ethanol and propolis) at a dose of 0 , 5 ml / kg of animal weight. Animals of the third group received intravenous trypinil at a dose of 5 ml / 100 kg of body weight (2.36 g / 300 kg body weight), together with the preparation of CGEP at a dose of 0.5 ml / kg body weight of the animal and tetracycline solution - oxy-100 at a dose of 5 ml / 100 kg body weight. Drugs were injected once a day for two days in a row. After 14 days, animals were re-enriched and repeated microscopy of blood smears. As can be seen from Table 2 data, in animals of the first group, there was a presence of up to 15 % babesia in erythrocytes. In animals of the second group, the presence of babesia was noted in 8 % of red blood cells. And in animals of the third group of babesia were detected only in 1-2 % of red blood cells. In a repeated study of blood serum for leptospirosis in PMA after the first regimen of treatment in all horses, there was a decrease in antibody titres to 1: 100. In the second group of animals, antibodies decreased to 1:50. In the third group of animals, the titre of antibodies in the serum was 1:20. At the same time, in the third group, 2 weeks after the treatment, high levels of hemoglobin, hematocrit, total protein, immunoglobulins, the number of red blood cells and a decrease in the number of leukocytes and titres of leptospirosis antibodies were noted (P < 0.001). That is, the blood indices corresponded to the indicators of healthy horses. In this household it was decided to carry out treatment and prophylactic treatment with triponil at the rate of 1 ml of the preparation for 30 kg of body weight, additionally injected with oxy-100 at a rate of 5 ml / 100 kg of body weight and intravenous SGEP at a dose of 0.5 ml / kg of body weight once for two days in a row for a day. In addition, all horses were treated externally with ectosan powder "Brovafarma". Such complex treatment helps to improve the economy in a coherent course of leptospirosis with babesiosis in horses.

Key words: horses, leptospirosis, babesiosis, compatible course of diseases, hematological and serological parameters of blood, methods of treatment