Дослідження на вміст Staphylococcus Spp. молока від хворих на субклінічний мастит корів

Ю. Ю. Вішован



Y. Y. Vishovan


Masking cows is an acute problem for the dairy industry around the world. The disease takes various forms, each of which has its own characteristics on any individual dairy farm. One of the most dangerous diseases for dairy herds is staphylococcal mastitis. With the secretion of the mammary gland, the pathogen spreads in the environment and is in the air, water, soil, and on various items of use for quite some time, which may be pathogens for susceptible individuals. It is important to take into account that during outbreaks of food toxicoinfections among humans, the most frequent factors of transmission of staphylococci are contaminated products of livestock (milk, meat, fish). The purpose was to investigate the staphylococcal content of milk, selected from healthy and patients with subclinical mastitis of cows.

160 samples of milk from healthy and patients with subclinical mastitis of cows from 2 farms of Kiev and farms from Khmelnytsky regions were investigated. The research of samples and the allocation of pure culture was carried out by the Drigalsky method on a dense nutrient medium of the Beard-Parker agar. Agar Beard-Parker agar medium is recommended for the selection and counting of coagulase-positive staphylococci from food and other materials. This medium is cited as the agar medium O in the European Pharmacopoeia and is recommended for the selection and calculation of coagulase-positive Staphylococcus aureus. It was developed by Baird-Parker and based on the tellurite-glycine formula to isolate coagulase-positive Staphylococcus aureus from food. Types of Staphylococcus are commonly used food, milk, pharmaceutical and cosmetics related condoms. The Bird-Parker environment is mentioned as the best medium for selective detection of coagulase-positive and intestinal-toxic Staphylococcus. Coagulase-positive staphylococci can grow and multiply in cosmetic products. Therefore, they are checked for coagulase-positive staphylococci using standard microbiological methods. It has been established that this medium less inhibits S. aureus than other media, while remaining more selective. Agar Beard-Parker contains a meat extract, a yeast extract and a pancreatic gaseous casein that provides essential minerals, vitamins, nitrogenous substances and other growth factors. Sodium pyruvate protects damaged cells and helps to restore them, as well as stimulates the growth of staphylococcus aureus, while retaining selectivity. Lithium chloride and potassium telluride suppress most of the contaminating microflora other than S.aureus. The recovery of tellurite is a characteristic of coagulase-positive staphylococci, and it gives colonies a black color. Glycine, pyruvate stimulate Staphylococcus growth. When the egg yolk is added, the medium becomes yellow and opaque. Glycine neutralizes aldehyde, while egg yolk neutralizes phenolic compounds, if any, in the test samples. Adding egg yolk, in addition to enrichment, also helps in the identification process, demonstrating lecithin activity (egg yolk reaction). Staphylococci containing lecithinase, spread egg yolk and form transparent areas around colonies. Transparent zones and gray-black colonies in this environment are diagnostic for coagulase-positive staphylococci. When further incubation around the colonies formed an opaque zone, which may be associated with lipolytic activity. The identity of the staphylococcus aureus isolated on the Beid-Parker agar should be confirmed by a reaction with coagulase and a deoxyribonucleic acid test.

A total of 160 samples were investigated; Staphylococcus cultures were isolated in 59 samples, which is 36.9%, including microorganisms of the genus Staphylococcus spp from 14 samples of milk from cows suffering from subclinical mastitis from the Khmelnytsky region farm. was detected in 2 cases, which is 14.29%. In 16 samples of milk of patients with subclinical mastitis of cows from the economy of the Kiev region, staphylococci were also detected in 2 cases, which is 12.5%. In another farm of the Kiev region of the sample of 130 samples staphylococci were found in 41 cases, which is 31.5%. Of these, from clinically healthy animals, staphylococci were isolated in 31.7% of cases and from patients with subclinical mastitis in 68.3%.

Of the 160 milk samples from clinically healthy and patients with subclinical mastitis of cows, 59 cultures of microorganisms identified as representatives of the genus Staphylococcus were identified. The directions of our further research will be: the next species identification, study of the biological properties of staphylococci - the reaction of plasma coagulation, fermentation of mannitol; determination of antibiotic resistance of isolated strains, ability to biofilm formation.


Повний текст:




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