Influence of heavy metals on antioxidant system and biochemical indexes in rats

I. V. Kalinin, V. A. Tomchuk, V. A. Gryshchenko


The study was undertaken to examine the effect of heavy metals on antioxidant system and biochemical indexes in the organism of rats. The influence of heavy metals on indexes of antioxidant system of lipid peroxidation processes indices, hydroperoxides and thiobarbituric acid (TBA)-active products and increase of activity of the antioxidant system enzymes (AOS) glutathioneperoxidase, glutationereductase, catalase and superoroxidedismutase in blood of rats was determined. It is established, that under the action of heavy metals in blood and liver of rats functions more intensively AOS. The study of enzyme activity showed the activation of the latter under conditions of heavy metal intoxication in 1.5 - 2.0 times (depending on a heavy metal), compared with control.  We found that in intoxicated rats in all experimental groups, levels of total and direct bilirubin, creatinine, urea, compared with intact animals was increased. However, a decrease in the content of albumin, total protein, cholesterol and triglycerides was also found in all experimental groups, in relation to intact rats. At the action of heavy metals rises activity of α-amylase total, lactate dehydrogenase and concentration glucose in the blood of rats. According to the results of studies in intoxicated animals, compared with the intact group, there was a change in the cation-anion pool, in particular, a tendency to decrease the content of sodium and inorganic phosphorus and increase chlorides, magnesium, calcium and potassium.

Keywords: rats, blood; liver; copper; zinc; cadmium; lead; antioxidant system.

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