Knee osteoartrosis modeling in dogs
DOI:
https://doi.org/10.31548/bio2018.05.021Keywords:
Pond-Nuki, histology, joint, osteoarthritis, cartilage tissue, pathology, dogs.Abstract
The article presents the results of conducted researches on osteoarthrosis modeling in dogs using the Pond-Nuki surgical method. The method of surgical operation and pereparing of histopreparations is described. The presence of expressive microscopic changes in tissue is described; and macroscopic changes in the joint. Its noted that osteoarthritis of the knee joint of dogs in the articular cartilage of the femur reveals the destruction of the surface layers of the articular cartilage, the disordered location of chondrocytes, the destruction and lysis of the part of chondrocytes, as well as the reduction of the content of glycoproteins in the intercellular substance.
The knee osteoarthrosis modeling in dogs using the Pond-Nuki method was performed and the obtained macroscopic and microscopic changes were described. Experimental animals were dogs. Control was provided by dogs who did not receive treatment. The results of comparative histological evaluation of articular cartilage changes in operated and non-operated knee joints in the control group of dogs showed that the histological changes appear only one month after the operation (changes in articular cartilage of operated knee joints were 1.6 ± 0.11, p <0 , 0001, and in the articular cartilage of the nonoperated knee joints 0.073 ± 0.005).
The degree of destructive changes in the joints of different dogs was relatively similar. The obtained data confirm our conclusion about the adequacy of the revealed structural changes in osteoarthritis. Osteoarthrosis of the knee joint in dogs is characterized by expressive microscopic changes in the cartilaginous tissue that covers the articular surface of the femur. It was discovered the destruction of the surface layers of the articular cartilage of the femoral bone and, the disordered location of chondrocytes ,including the destruction and lysis of the part of chondrocytes, as well as the reduction of the content of glycoproteins in the intercellular substance was found.
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