Іnfluence of glyfosat-resistant genetically modified soy and Roundup herbicide on the level of some metabolites of nitrogen exchange in rats

Authors

  • I. V. Chorna National Technical University «Kharkiv Polytechnic Institute» , Національний технічний університет «Харківського політехнічного інституту»

DOI:

https://doi.org/10.31548/bio2019.03.001

Keywords:

urea, creatinine, uric acid, nitrogen metabolism, total protein, genetically modified soy, traditional soy

Abstract

The creation and use of transgenic plants in livestock and food industries are a source of great concern and debate in society. Effects of transgenic plants on humans and animals have not yet been sufficiently studied, which necessitates the study of the influence of genetically modified plants on metabolic processes in animals. Research on the effects of genetically modified plants on several generations of rats is important for assessing the safety of their use. Today, among all genetically modified plants, soybeans are most commonly used with the new sign "Roundup Ready", which are resistant to the "Roundup" herbicide that is widely used throughout the world. That is why the study of both the individual and combined effects of both of these factors on the organism of animals is very relevant today.

The aim of the experimental research was to study the effect of glyphosate resistant genetically modified soybeans untreated and treated with the "Roundup" herbicide and the effect of the herbicide itself on the level of some metabolites of nitrogen metabolism in the urine of three generations of rats. The object of the study was the rats of the Wistar line weighing 190-220 g, at the age of 4 months. Five groups of animals were formed, each with 16 rats (8 females and 8 males). Group I - intact animals (kept on a standard vivarium diet); Group ІІ - animals, in which 20-26% of the standard dietary nutrition diet was replaced by traditional soybeans; Group III - rats, in which 20-26% in the standard diet is replaced by genetically modified soybean, not treated with a "Roundup" herbicide; ІV group - rats, ration of which contained 20-26% of genetically modified soybean, treated with herbicide "Roundup"; V group - rats, who consumed herbicide with drinking water in a dose (0.003 μg/kg of animal weight). After 42 days, the rats were mating and receiving the next generation; another generation of rats was similarly obtained.

Data from experimental studies showed a decrease in the level of nitrogen metabolites (creatinine and urea) and increased uric acid and protein levels in urine of all three generations of rats in groups IV and V. For the traditional and transgenic soybeans used by unprocessed herbicides, the level of metabolites of nitrogen metabolism increases slightly. The revealed changes indicate a violation of the liver and kidneys, and the disturbance of the protein metabolism, which may lead to various pathological conditions in the body of animals.

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Published

2019-08-23

Issue

Vol. 11 No. 3-4 (2019)

Section

Biology

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