Readability indicators of water regime and content of nucleic acids in leaves of plant genus Aesculus L. action by horse-chestnut miner moth

Authors

  • O. S. Penteliuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • I. P. Grigoryuk National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/bio2016.05.001

Abstract

Mechanisms of plant resistance to stressful environmental factors are the result of complex changes in the metabolic processes functionating at various levels of their organization. The intensity and trend of growth and development of plants depends on the parameters of the water regime and the content of nucleic acids (NA). It is established that the number of deoxyribonucleic and ribonucleic acids (DNA, RNA) varies depending on the individual development of plants. The aim of work was to determine the parameters of daily water deficiency (WD), the number and ratio of RNA to DNA, as components of biosynthetic processes intensity and stability of plant genus Aesculus L. against horse-chestnut miner moth (HCMM) (Cameraria ochridella Deschka et Dimič). The objects of research is served optimally developed and coeval leaves of the lower, middle and upper tiers of plant species genus Aesculus L. 20-30-years of age, who are growing in the green zone of Kiev’s megapolis were selected during the massive damage to plants HCMM. The close correlation was shown between indicators of water regime, RNA and DNA content and degree of stability of plant genus Aesculus L. against HCMM in organismal level. If the biochemical ratio of RNA to DNA in the leaves in the fruit phase is 1,10-1,40, the species of the genus Aesculus L. characterized by low intensity biosynthetic processes and resistance to HCMM, 4,29-4,56 - secondary and 8,89-10,0 - high. The ratio of RNA to DNA was recommended as diagnostic marker or as indication of biological stability of species to genus Aesculus L. HCMM.

References

Grigoryuk, I.A., Tkachev, V.I., Savinskiy, S.V., Musienko, N.N. (2003). Sovremennye metody issledovaniya i otsenki zasukho- i zharoustoychivosti rasteniy [Modern methods of investigation and assessment of drought and heat resistance of plants]. Кyiv, Naukovyy svіt, 139.

Hryhoriuk, I. P., Mashkovska, S. P., Yavorovskyi, P. P., Kolesnichenko, O.V. (2004). Biolohiia kashtaniv [Chestnut`s biology]. Kyiv, Lohos, 380.

Hryhoriuk, I.P., Luk’ianenko, T.L. (2015). Fiziolohichni i molekuliarni osnovy stiikosti vydiv roslyn rodu Aesculus L. proty kashtanovoi minuiuchoi moli [The physiological and molecular basis of plant resistance genus Aesculus L. against horse-chestnut miner moth]. Kyiv, Komprynt, 174.

Djevidson, Dzh. (1976). Biohimija nukleinovyh kislot [The biochemistry of nucleic acids]. Moscow, Russia: Mir, 371.

Konarev, V. G., Ahmetov, R. R., Eleakova, T. N., Chekman, I. F. (1964). Metody opredelenija sostojanija ribonukleinovyh kislot v kletke [Methods of determining the state of ribonucleic acids in the cell]. 2-ja nauchnaja konferencija po nukleinovym kislotam u rastenij. [2nd Scientific Conference on nucleic acids

in plants]. Moskow, Russia: Nauka, 145-151.

Ermakov, A. I. ed. (1972). Metody biohimicheskogo issledovanija rastenij [Methods of Biochemical Plant Research]. Lviv: Kolos, 456.

Raha, S., Merante, F., Proteau, Y., Reed, L.K. (1990). Stimultaneus isolation of total cellular RNA

Downloads

Published

2017-05-11

Issue

Vol. 8 No. 5-6 (2016)

Section

Biology

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).