Сomparison of efficiency of immunobiosensors based on gold and ZnO nanoparticles for analysis of spermine levels in in vitro cell culture

Authors

  • M. P. Prylutskyi National University of "Kyiv-Mohyla Academy" , Національний університет «Києво-Могилянська Академія»
  • M. F Starodub National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. I. Fedelesh-Gladynets National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/biologiya2019.03.036

Keywords:

polyamines, spermine, biosensors, nanoparticles, transducer

Abstract

Nowadays, for diagnostics of oncological diseases, it is necessary to develop the newest methods, aimed primarily at fast, accurate, mobile and low cost analysis. The extraordinary complexity of carcinogenesis and the difficulties of early detection of the disease pose a primary concern - the choice of an optimal diagnostic methods. Such methods may include the development and use of immunobiosensors for the analysis of low molecular weight markers of cancer. In a role of such markers could become polyamines which are low molecular weight substances presented in cells of living organisms and can affect a wide range of cellular functions, one of which is proliferation. Increased levels of polyamines can often be a sign of the beginning of the process of malignancy, and therefore can be used as a markers for the diagnostics of oncological diseases. Immunobiosensors possess certain physical and biological properties, and are capable to interact with the specific antigens and detect them in biological fluids. This article presents the results of studies pointed on the comparison of the effectiveness of analytical capabilities of immunobiosensors with different types of transducers, namely, a transducer covered with particles of gold, and that is covered with zinc oxide nanoparticles. The obtained data allow to observe a gradual increase in the concentration of polyamines, both in solution and in suspension of cells. It has been determined that the biosensor which was covered with gold particles detected the presence and concentration of polyamines, both in solution and in the suspension of MCF-7 breast cancer cells better comparing with zinc oxide transducer, and capable to detect spermine in concentrations ranging from 5 to 100 ng/ml and in the concentration of MCF-7 cells from 100 to 500 cells/ml.

Author Biographies

  • M. P. Prylutskyi, National University of "Kyiv-Mohyla Academy", Національний університет «Києво-Могилянська Академія»

    провідний спеціаліст кафедри лабораторної діагностики біологічних систем

  • M. F Starodub, National University of Life and Environmental Sciences of Ukraine
    завідувач кафедри молекулярної біології, мікробіології та біобезпеки, доктор біологічних наук, професор
  • M. I. Fedelesh-Gladynets, National University of Life and Environmental Sciences of Ukraine
    кандидат сільськогосподарських наук, доцент кафедри  кафедри молекулярної біології, мікробіології та біобезпеки

References

Casero, J. R., Murray, T. S., Pegg, A. E. (2018). Polyamine metabolism and cancer: treatments, challenges and opportunities. Nature Reviews Cancer, 18(9), 681–695. doi:10.1038/s41568-018-0050-3

Casero R. A (2006). Targeting polyamine metabolism and function in cancer and other hyperproliferative diseases. Nature Reviews. Drug Discovery. 6, 373–390. doi:10.1038/nrd2243.

Çelik, V. K. Kapancık S., Kaçan T., Kaçan S. B., Kılıçgün H (2017). Serum levels of polyamine synthesis enzymes increase in diabetic patients with breast cancer. Endocrine Connections. 6(8). 574–579. doi:10.1530/EC-17-0137.

Criss, W. E. A review of polyamines and cancer (2003). Turkish Journal of Medical Sciences. 33 (4), 195–205.

Gerner, E.W. Meyskens F. L. (2004). Polyamines and cancer: old molecules, new understanding. Nature Reviews. Cancer. 4, 781–789. doi:10.1038/nrc1454.

Guerra, G. P., Rubin, M. A., Mello, C. F. (2016). Modulation of learning and memory by natural polyamines. Pharmacological research. 112, 99–118. doi:10.1016/j.phrs.2016.03.023.

Levêque, J. Foucher F., Bansard R. Havouis R., Grall J-Y., Moulinoux J-P (2000). Polyamine profiles in tumor, normal tissue of the homologous breast, blood, and urine of breast cancer sufferers. Breast Cancer Research and Treatment. 60 (2), 99–105. doi:10.1023/A:1006319818530.

Nowotarski, S. L. Woster P. M., Casero R. A. (2013) Polyamines and cancer: implications for chemotherapy and chemoprevention. Expert Reviews in Molecular Medicine. 15 (3), 1–21. doi:10.1017/erm.2013.3.

Reddy, P. J., Sadhu, S., Ray, S., Srivastava S. (2012). Cancer Biomarker Detection by Surface Plasmon Resonance Biosensors. Clinics in Laboratory Medicine. 32(1), 47–72. doi:10.1016/j.cll.2011.11.002.

Soda K. (2011) The mechanisms by which polyamines accelerate tumor spread. Journal of Experimental & Clinical Cancer Research. 30 (1), 95–100. doi:10.1186/1756-9966-30-95.

Thomas, T., Thomas, T. J. (2003). Polyamine metabolism and cancer. Journal of cellular and molecular medicine. 7(2), 113–126. doi:10.1111/j.1582-4934.2003.tb00210.x

Downloads

Published

2019-09-30

Issue

Vol. 10 No. 3 (2019)

Section

Статті

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).