Storage of erythrocyte mass of horses and control of its sterility

Authors

  • D. V. Tarnavskyi National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • O. V. Egorov National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • M. O. Malyuk National University of Life and Environmental Sciences of Ukraine image/svg+xml
  • G. V. Kozlovskaya National University of Life and Environmental Sciences of Ukraine image/svg+xml

DOI:

https://doi.org/10.31548/dopovidi2022.06.008

Keywords:

erythrocyte mass of horse blood, microbial contamination, sterility of donor blood

Abstract

Currently, blood transfusion should be considered as a tissue transplantation operation with all the consequences - possible rejection of cellular and plasma components of blood, development of allosensitization to antigens of blood cells and plasma proteins, as well as, in the case of an immunodeficient patient, the possible development of a life-threatening reaction "Transplant against host".

Erythrocyte mass is a transfusion medium containing at least 70% erythrocytes, which is optimal in the treatment of anemic syndrome. At equal volumes compared to whole blood, the erythrocyte mass contains a larger number of erythrocytes, but significantly less citrate, products of cell decay, cellular and protein antigens, and antibodies. Transfusions of erythrocyte mass occupy a prominent place in transfusion therapy, which aims to fill the deficiency of erythrocytes in anemic conditions.

We studied 5 samples of preserved erythrocyte mass from clinically healthy horses. Blood was collected by a closed method followed by separation of the erythrocyte mass by centrifugation. The mass was stored in a refrigerator at a temperature of 2-6°C for 30 days.

Sowing of each sample was carried out in the thickness of the nutrient medium. Cultures in a thioglycol medium and control tubes were incubated in a thermostat at a temperature of 20-25°C and at a temperature of 30-35°C, with a Sabouraud medium at a temperature of 20-25°C. The term of incubation of crops in a thermostat in both nutrient media was 14 days.

According to the results of bacteriological examination of samples of erythrocyte mass of horses after their storage, not a single non-sterile sample was found.

Therefore, the method of blood collection using closed systems is reliable and makes it possible to receive donor blood and blood components without loss of sterility during a long storage period.

References

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Published

2022-12-29

Issue

No. 6/100 (2022)

Section

Veterinary medicine, quality and safety of livestock products

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