USING OF P40 TECHNIQUE FOR BRAIN SHEET PLASTINATION
Abstract
P40 plastination technology is a specific technique for the tissue preservation and production of brain slices. The original version of the processing has several phases: fixation, dehydration, slicing, impregnation, hardening and grinding. For achieve of high quality anatomical specimens with classic technique requires two months. The aim of this research is to reduce the time of production process. Fixation: We use 14 brains that are fixed in 10 % water mixture of for-maldehyde for 1 month after perfusion of whole cadaver. The perfusion is a best method for cadaver fixation. Slicing: We do not set whole brains in acetone in dehydration step, but put them at minus 25 oC freezer for 24 hours. Аll brains hardened by low tem-perature and it is easily to cut with a machine cutter and get a slice thickness 4–5 mm.
Dehydration: Slices 4–5 mm thick does not require one month to dehy-dration. 2–4 days, depending on the thickness thereof is sufficient. Acetone is an ideal dehydrator in all plastination techniques. We put brain slices on a metal grid and set them in cold (-25 oC) first acetone (100 % purity) bath. The next day must to measure the purity of the acetone, and if necessary replacing it with a new acetone (100 % purity). The process is completed in 2–4 days.
The P40 brain slices are semitransparent, durable and safely for students and lectures. They represent an excellent sectional anatomy of the brain. A comparison between slices, made at a different position of the flat camera, is found that when operating with a horizontal chamber, results are better – there is no displacement of the compositions, the polymer being illuminated better and no cracks. We have also discovered that the bubbles are removed more easily in the vertical position of the camera. Therefore, we recommend air bubbles to remove in a vertical position of the flat camera but the illumination to do in a horizontal position, if possible – on both sides of the preparation.
Аnatomy, plastination, P40, brain, slices, fixation, dehydration, impreg-nation.
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