Контамінація українського бджолиного обніжжя грибами роду Fusarium

Authors

  • О. О. Застулка
  • О. М. Якубчак
  • Л. О. Солодка

Abstract

MICROSCOPIC FUNGI (THE GENUS FUSARIUM) CONTAMINATION OF UKRAINIAN BEE POLLEN

 O. Zastulka, O. IYakubchak, L. Solodka

 

 Annotation. The aim of our study was to identify in samples of bee pollen harvested in Ukraine in the years 2015-2016, potentially toxigenic fungi of the genus Fusarium. 7 samples were examined in 2015 and 15 samples in 2016 that corresponded to the national standard for acidity 2% aqueous solution and the content of flavonoids. Bee pollen in dilution 1:10 and 1:20, several times, by 2-3 repetitions was deep seeded on agar Capek and agar Saburo with glucose and was grown at a temperature of 25°C for 5-7 days. In some parties of bee pollen were found fungi of the genus Fusarium in an amount of 20-83 CFU per 1 g (2015 year) and 15-102 CFU/g (2016 year). By organoleptic and physico-chemical parameters bee pollen were performed to the national standart 3127-95. These fungi are able to release a number of toxins and therefore are potentially dangerous components of dietary supplements for humans and animals. It is advisable to allocate this type of fungi in carrying microbiology of quality pollen parties and it’s recommended also to bring this procedure used into the state normative documents.

Keywords: bee pollen, dietary supplement (BAA), microscopic fungi colony forming units (CFU), cultural and morphological characteristics of fungi of the genus Fusarium, toxicity

References

References

Tutelyan, V. A., Kravchenko, L.V. (1985). Mycotoxins [Mycotoxins]. Moscow. Moscow State University, 295.

Bilai, V. I., (1977). Fusarium [Fusariums]. Kiev. Naukova Dumka, 256.

Raillo, A. I. (1950). Fungi of the genus Fusarium [ Fusarium fungi]. – Moscow. Gos. Publishing house of agricultural literature, 340.

Marasas,W., P. Nelson, P., Tousson, T., (1984). Toxigenic Fusarium Species: Identity and Mycotoxicology [Toxigenic Fusarium Species: Identity and Mycotoxicology] Pennsylvania. Pennsylvania State University Press, 278.

Platonova, Yu. V., Surin, N.A. (2004). Geography of fungi of the genus FUSARIUM (Literary Review) [Geography of fungi of the genus FUSARIUM (Literary review) ]. Fundamental research. No. 4.95-97.

Grigoryan, K. M., Brindza, J., Sargsyan, M.P, Hakobyan, L. L. K voprosu o kontamynytsyy obnozhkovoi pыltsы hrybamy y mykotoksynamy [Elektronnyi resurs] [On the issue of contaminant pollen pollen with fungi and mycotoxins [Electronic resource] International peer-reviewed scientific journal "Immunopathology, Allergology, Infectology" - Mode access to the resource: www.immunopathology.com/en/article.php?download_pdf=24.

Leslie, J. B. Summerell B.The Fusarium Laboratory Manual – 2121 State Avenue, Ames, Iowa 50014, USA: Blackwell Publishing Professional. 387.

Tsuneo, W. (2000). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species.—2nd ed. CRC Press LLC, 486.

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Published

2018-05-16

Issue

No. 265 (2017)

Section

Article

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