BIOCHEMICAL CHANGES IN SERUM OF RABBITS WITH EXPERIMENTAL MECHANICAL DAMAGE OF THE BONE TISSUE AFTER APPLICATION OF ALLOGENEIC MESENCHYMAL STEM CELLS

Authors

  • T. Savchuk National University of Bioresources and Natural Resources of Ukraine , National University of Bioresources and Natural Resources of Ukraine
  • A. I. Mazurkevych National University of Bioresources and Natural Resources of Ukraine , National University of Bioresources and Natural Resources of Ukraine
  • M. O. Maliuk National University of Bioresources and Natural Resources of Ukraine , National University of Bioresources and Natural Resources of Ukraine

Abstract

Summarizing the results of previous studies on the nature of
the optimization and stimulation of reparative osteogenesis and the potential of
studying this problem, our goal was to trace the biochemical changes during
250
250the process of reparative osteogenesis in experimental bone for the
introduction of allogeneic mesenchymal stem cells.
Damage of the bone tissue was modeled in rabbits 3 months of age of
the chinchilla breed, in the middle third of the diaphysis of the tibia. Blood
sampling was performed from the jugular vein before surgery and at 3, 7, 14,
21, 28 days after injury. Biochemical studies were made using biochemical
analyzer RT-9600. The obtained results were processed statistically.
The results of studies of biochemical changes, namely, content of Cа, P
and alkaline phosphatase activity in blood serum of rabbits after an
experimental mechanical damage of the bone tissue and the application of
allogeneic mesenchymal stem cells. It was found that after the introduction of
allogeneic mesenchymal stem cells for the regeneration of bone tissue, where
the maximum increase in the content of Cа, P and alkaline phosphatase
activity coming soon, soon comes back to normal compared to control group
animals, starting with 3 day and ending on 28 day of the study. Which
indicates that the stem cells is able to differentiate into osteogenic direction
and influence the processes of bone tissue regeneration. And for the
introduction of allogeneic mesenchymal stem cells in place of experimentally
injured bone tissue regeneration processes are more intense in contrast to the
introduced allogenic mesenchymal stem cells in the jugular.
The obtained data can be used to develop methods and means of
preventing possible complications in the recovery process of damaged bone
tissue, as well as for further experimental studies.
Keywords: reparative osteogenesis, callus, mesenchymal stem
cells, alkaline phosphatase, calcium, phosphorus, blood, serum, bone

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Published

2019-03-05

Issue

No. 285 (2018)

Section

Article

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