Autologous stem cells therapy in horses and dogs with chronic osteoarthritis
DOI:
https://doi.org/10.31548/ujvs2020.02.001Abstract
Abstract. One of the fastest growing areas of veterinary medicine over the past decade has been and still is the regenerative medicine. It focuses on the reconstruction of damaged tissues/organs to restore their physiological and functional performance. The aim of study was the use of cells called mesenchymal stem cells (MSC) as an alternative treatment method of osteoarthritis. Twelve dogs have been chosen for experiment aged 5 to 11 years. All animals were diagnosed with the degeneration of the elbow joint. The dogs were divided into 2 groups: 8 dogs formed the test group and the remaining 4 dogs formed the control group. The dogs from the first group were treated with autologous stem cells. The dogs in the control group received non-steroidal anti-inflammatory drugs (NSAIDs) − mavacoxib for a period of 6 months. Clinical examination on the first day of the experiment showed signs of lameness of the 3rd and 2nd degree in all 12 dogs. The clinical tests performed on day 180 in the control group showed signs of gait disturbance at the unchanged level. The dogs undergoing treatment with the use of autologic stem cells did not show any movement disorders. The obtained results allow drawing the conclusion that the combination of the lowest concentrations of metamizol may significantly increase the cytophysiological activity of the stem cells accompanied by the lack of morphological changes. The obtained data can be a valuable source of information for practicing veterinary doctors who introduce stem cells to treat musculoskeletal system diseases in both small and large animals.
Keywords: stem cells, mesenchymal stem cells (MSC), osteoarthritis, non-steroidal anti-inflammatory drugs (NSAIDs), mavacoxib
References
Barry, F. P. & Murphy, J. M. (2004). Mesenchymal stem cells: clinical applicatons and biological characterizaton. Stem Cells, 36:568−584.
https://doi.org/10.1016/j.biocel.2003.11.001
Black, L. L., Gaynor, J., Adams, C., Dhupa, S., Sams, A. E., Taylor, R., Harman, S., Gingerich, D. A. & Harman, R. (2008). Effectof intraartcular injecton of autologous adiposederived mesenchymal stem and regenerative cells on clinical signs of chronic osteoarthritis of the elbow joint in dogs. Vet Ther, 9:192−200.
Burk, J., Ribitsch, I., Gittel, C., Juelke, H., Kasper, C., Staszyk, C. & Brehm, W. (2013). Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources. Vet. J., 195:98-106.
https://doi.org/10.1016/j.tvjl.2012.06.004
Dimitrieva, R. I., Minullina, I. R., Bilibina, A. A., Tarasova, O. V., Anisimov, S. V. & Zaritskey, A. Y. (2012). Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: differences and similarities. Cell Cycle, 11:377-383.
https://doi.org/10.4161/cc.11.2.18858
Egenvall, A., Lönnell, C., Johnston, C. & Roepstorff, L. (2010). Orthopaedic health status of horses from 8 riding schools − a pilot study. Acta Vet. Scand., 52:2-8.
https://doi.org/10.1186/1751-0147-52-50
Gonzalez, M. A., Gonzalez-Rey, E., Rico, L., Buscher, D. & Delgado, M. (2009). Adipose derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Gastroenterology, 136:978-989.
https://doi.org/10.1053/j.gastro.2008.11.041
Guercio, A., Di Marco, P., Casella, S., Cannella, V., Russotto, L., Purpari, G., Di Bella, S. & Piccione, G. (2012). Production of canine mesenchymal stem cells from adipose tissue and their application in dogs with chronic osteoarthritis of the humeroradial joints. Cell Biol Int, 36:189−194. https://doi.org/10.1042/CBI20110304
Hackett, C. H. (2013). Assessing the function of mesenchymal stromal cells: all that glitters is not gold. Vet. J., 195:10-11.
https://doi.org/10.1016/j.tvjl.2012.09.017
Holloway, H., Robinson, A. & Mailand, T. P. (2012). Benefits of continuous long-term treatment of canine degenerative joint disease with carprofen. Rev. Méd. Vét., 163:95-104.
Lai, R. C., Chen, T. S. & Lim, S. K. (2011). Mesenchymal stem cell exosome: A novel stem cell-based therapy for cardiovascular disease. Regen. Med, 6:481−492.
https://doi.org/10.2217/rme.11.35
Marycz, K., Toker, N. Y., Grzesiak, J., Wrzeszcz, K. & Golonka, P. (2012). The therapeutic effect of autogenic adipose derived stem cells combined with autogenic platelet rich plasma in tendons disorders in horses in vitro and in vivo research. J. Anim. Vet. Adv., 11:4324−4331.
Mason, C. & Dunnill, P. (2008). A brief definition of regenerative medicine. Future Medicine, 3:1−5. https://doi.org/10.2217/17460751.3.1.1
Minguell, J. J., Alejandro, E. & Paulette, C. (2001). Mesenchymal stem cells. Exp. Biol. Med., 226:507−520. https://doi.org/10.1177/153537020122600603
Ratajczak, J., Kucia, M., Mierzejewska, K., Marlicz, W., Pietrzkowski, Z., Wojakowski, W., Greco, N. J., Tendera, M. & Ratajczak, M. Z. (2013). Paracrine roangiopoietic effects of human umbilical cord blood-derived purified CD133+ cells-implications for stem cell therapies in regenerative medicine. Stem Cells Dev, 22:422−430. https://doi.org/10.1089/scd.2012.0268
Sandell, L. J. (2012). Etiology of osteoarthritis: Genetics and synovial joint development. Nat. Rev. Rheumatol, 8(2):77−89. https://doi.org/10.1038/nrrheum.2011.199
Strem, B. M., Hicok, K. C., Zhu, M., Wulur, I., Alfonso, Z., Schreiber, R. E., Fraser, J. K. & Hedrick, M. H. (2005). Multipotential differentiation of adiposederived stem cells. Keio J. Med., 54:132−141. https://doi.org/10.2302/kjm.54.132
Tuglu, M. I., Ozdal-Kurt, F., Koca, H., Sarac, A., Barut, T. & Kazanc, A. (2010). The contribution of differentiated bone marrow stromal cell-loaded biomaterial to treatment in critical size defect model in rats. KafkasUniv Vet Fak Derg, 16:783−792.
Yasuda, K., Khandare, A., Burianovskyy, L., Maruyama, S., Zhang, F., Nasjletti, A. & Goligorsky, M. S. (2011). Tunneling nanotubes mediate rescue of prematurely senescent endothelial cells by endothelial progenitors: exchange of lysosomal pool. Aging, 3:597-608. https://doi.org/10.18632/aging.100341
Zhu, Y., Liu, T., Song, K., Fan, X., Ma, X. & Cui, Z. (2008). Adipose-derived stem cell: a better stem cell than BMSC. Cell Bioch. Funct, 26:664−675.
https://doi.org/10.1002/cbf.1488
Zuk, P. A., Zhu, M., Ashjian, P., De Ugarte, D. A., Huang, J. I., Mizuno, H., Alfonso, Z. C., Fraser, J. K., Benhaim, P. & Hedrick, M. H. (2002). Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell, 13:4279-4295.
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