Identification of the allelic variants of paddlefish (polyodon spathula) microsatellite dna

Authors

  • Kh. M. Kurta Українська лабораторія якості і безпеки продукції АПК
  • О. O. Malysheva Institute of Veterinary Medicine the National Academy of Agrarian Sciences of Ukraine
  • B. O. Hrishyn The Institute of Fisheries of the National Academy of Agrarian Sciences
  • А. A. Getya National University of Life and Environmental Sciences of Ukraine
  • L. M. Shynkarenko Українська лабораторія якості і безпеки продукції АПК
  • V. H. Spyrydonov Institute of Veterinary Medicine the National Academy of Agrarian Sciences of Ukraine

DOI:

https://doi.org/10.31548/bio2017.05.007

Abstract

The microsatellite DNA assay of paddlefish (Polyodon spathula) and the genetic polymorphism of the investigated group of fish (n = 38) were performed in this paper for the following DNA-markers: Psp12, Psp21, Psp26 and Psp28. According to the microsatellite analysis, 24 allelic variants were identified for all loci. The Psp26 and Psp28 loci were the most polymorphic (7 allelic variants), and the Psp21 locus was the least polymorphic (4 allelic variants). For the Psp12 loci two new allelic variants of 214 bp (5,26%) and 216 bp (34,21%) were identified, and for the Psp26 loci one new allelic variant 164 bp (7,90 %) was identified. According to population calculations, it was found that in this group of fish predominates individuals with heterozygous genotypes and a fairly high level of genetic variability is observed.

Keywords: paddlefish (Polyodon spathula), microsatellites, DNA markers, loci, allele, polymorphism, genetic structure

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Published

2018-02-22

Issue

Vol. 9 No. 5-6 (2017)

Section

Biology

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