Method of assessment of influence of exogenous biostimulators on rooting of stem cuttings

Authors

DOI:

https://doi.org/10.31548/bio2019.01.014

Keywords:

stem cuttings, comprehensive rooting assessment, exogenous biostimulators, plant rooting hormones, rhizogenic ability, integrated index of rooting, Thujopsis dolabrata

Abstract

Because of creation the optimal rooting conditions, the issues of improving the humidification, letting in the light to plants in the greenhouse, its structural features etc. are relevant. Under the conditions of complying with the main technological requirements, in order to increase the plant rooting efficiency, the use of exogenous biostimulants (plant rooting hormones) becomes particularly important.

Objective – to develop and test the method of integrated assessment of the results of rooting of stem cuttings in experiments using exogenous biostimulators. The object of the research – rhizogenic ability of stem cuttings of phytological introduced species. Subject of researches – process of rhizogenesis in connection with the testing of exogenous biostimulators (biologically active substances) on the stem cuttings of hiba arborvitae (Thujopsis dolabrata Sieb. et Zucc.). There were used such methods as literary-analytical, physiological, and comparative, mathematical.

To research the objective the following tasks were set: 1) to carry out the experiments with the use of the method that involves taking into account the number of rooted stem cuttings; 2) to propose a scale for a quantitative and qualitative evaluation of the results (success) of rooting of stem cuttings; 3) to develop a method for determining the integrated (general) index of rooting of stem cuttings; 4) to carry out a comprehensive study of the rhizogenic capacity of hiba arborvitae using exogenous biostimulants.

The application the method of integrated assessment of the influence of exogenous biostimulators on the rhizogenic ability of stem cuttings allows to calculate an integrated (general) index of their rooting for each of the experimental variants. The proposed index takes into account the results of not only quantitative but also qualitative evaluation, which allows identifying the best variants of the use of exogenous biostimulators for the treatment of stem cuttings.

According to the results of the conducted research, a 4-point scale is proposed to apply for assessing the quality of rooting, as well as to evaluate the percentage of the results of the rooting of cuttings on a 6-point scale. For an integrated assessment of the effect of exogenous biostimulators on the rooting of stem cuttings of plants of a certain systematic or economic plant group, it is advisable to carry out the experiments in 3 stages.

The method for determining the integrated index of rooting is presented for the first time in the final report on the materials of the state scientific topic "To research the biological and technological basis of propagation of woody ornamental plants", No. of state registration 0106U0006299 (2006-2008). In the corresponding interpretation, the proposed method can be used in experiments to test different substrates, time of rooting of stem cuttings, methods of their preparation, greenhouse design, lighting, heating, etc., as well as to optimize the technology of care for cuttings during the time of rooting.

References

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Published

2019-02-28

Issue

Vol. 11 No. 1-2 (2019)

Section

Forestry

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