SENSITIVITY OF CULTURE ORNITHOBACTERIUM RHINOTRACHEALE TO ANTIBIOTICS ANTIBIOTICS

Authors

  • V. B. Dukhnytskyi National University of Life and Environmental Sciences of Ukraine
  • A. M. Tyshkivska National University of Life and Environmental Sciences of Ukraine

Abstract

Ornitobakterialnyrhinotracheitisreferstothegroupofrespiratorydisea ses,whichcurrentlyisoneofthemajorpoultryindustryproblemssinceleadtosignificantec onomiclossesduetolostproductivityincommercialandreproductivestage,deterioration ofthequalityofeggsanddecreaseincrementsandincreasedcullingofbroilers.Thecause ofthesediseasesisassociatedwiththeactionofvariousfactors,usuallymutuallyreinforci ngeachother. ThediseasehasbecomewidespreadinEurope, America, AsiaandAfrica. A largenumberofspeciesofpathogens, rapiddevelopmentofresistancetoantibiotics, difficultiesindiagnosis– thesefactorsmakeitdifficultforthebirdtocureforthisdisease. Isolationofthepathogenbyclassicalbacteriologicalmethodsisanextremelycomp lexprocess, andthereactionofsensitivitytoantibioticsof a circulatingspeciestakes a longtimeanddoesnotalwaysgivethedesiredresult. Butthechoiceoftherightchemotherapeuticdrugforfightingthepathogenisthemaincondi tionforsuccessfultreatmentandtheguaranteeofthewell-beingoftheherd. Toconductresearch, pathologicalmaterialfrom a clinicallyillpoultrywasselected, withsignsofinvolvementofrespiratoryorgans.

ThecausativeagentofOrnitobacteriumrinotrahealewasisolatedbybacteriologicalmeth odsofinvestigation. Forthediagnosis, moleculargeneticstudiesofwashingsfromthetrachea, usingthe PCR method, wereused. SensitivitytoantibioticsofOrnitobacteriumrinotrahealeculture, isolatedfrombroilerturkeys, wasdeterminedby a disk-diffusionmethod. Identificationoftheculturewasconfirmedbybiochemicaltests.Tosetupasensitivityr esponse,discswithantibioticswereused.Whenanalyzingtheantibioticsusceptibilit yofisolatedstrainsofmicroorganisms,optimalvaluesofthediameterofgrowthretard ation,characterizingthesensitivitylevelsforeachantibiotic,weretakenintoaccount. Accordingtoourstudies,thecirculatingtypeofagentappearedtobesensitivetoenrofl oxacin (27.0 mm zoneofgrowthinhibition), oxytetracycline (25.5 mm), amoxicillin (24.0 mm), doxycycline (22.3 mm), tylosin, andflortenipolutiamulin– thezoneofgrowthretardationof 22.0, 21.5 and 18 mm, respectively. Keywords: ornithobacteriosis, Ornithobacteriumrhinotracheale, tilmicosin, enrofloxacin, ORT

References

Tatarchuk, O. (2009) Borba s ornytobakteryozom v broilernomptytsevodstve

[Fighting with ornithobacteriosis in broiler poultry farming]. Veterynaryia, 12, 31–33.

(in Russian)

Hliebova, K. V., Maiboroda, O.V. (2015). Poshyrenniabakteriozivptytsi v

ptakhohospodarstvakhUkrainy [Distribution of bird bacteria in poultry farms of

Ukraine].Problemyzooinzh. ta vet. Med., 30 (2), 153–157. (in Ukrainian)

van Empel, P. C. M., Hafez H. M. (2005).Ornithobacteriumrhinotracheale: a

review. Avian Pathology, American Association of Avian Pathologists, 28, 217–227.

Wyffels, R., Homez, J. (2007). Pasteurellaanatipestifer isolatedfrom

respiratory lesions in partridges kept in captivity. VlaamsDiergeneeskundi g

Tijdschrift, 59, 105–106.

Ornithobacterium rhinotracheale Antibody Test Kit For the detection of

antibody to O.r. in Chickens and Turkeys. BioChek Poultry Immunoassays.

Van Empel, P.C.M. (1998). Ornithobacteriumrhinotracheale: current status and

control. In Proceedings of the 1st International Symposium on Turkey Diseases, Berlin,

–137.

van Empel, P., van den Bosch, N., LoeffenH. (1997).Identification and serotyping

of Ornithobacteriumrhinotracheale. Journal of Clinical Microbiology, 35, 418–421.

Cauwerts, K., Herdt, P., Haesebrouck, F. (2004). The effect of

Ornithobacteriumrhinotracheale vaccination of broiler breeder chickens on the

performance of their progeny. Avian Pathology, American Association of Avian

Pathologists, 31, 619–624.

Downloads

Published

2019-03-05

Issue

No. 285 (2018)

Section

Article

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).