Blood and acid composition of blood and biles in calves at enteropatology and application of milk phospholipides
DOI:
https://doi.org/10.31548/ujvs2019.04.005Keywords:
жовчні кислоти, кров, жовч, телята, ентеропатологія, репаративна терапія, фосфоліпіди молокаAbstract
Abstract. Bile acids are important constituents of bile, which is produced by the liver and ensures the complete digestion and assimilation of nutrients of a lipid nature in the small intestine. The development of enteropathology in newborn calves adversely affects the functional state of both the intestines and the liver. The purpose of this work was to determine the characteristic changes in the bile acid spectrum of blood and bile of calves who had had functional enteropathology, and to test the corrective effectiveness of the biologically active additive (BAA) «FLP-MD» reparative action based on milk phospholipids. Free and conjugated bile acids were examined in the blood and bile of calves using thin layer chromatography.
It has been established that calves who have been affected by enteropathology have a decrease in the biosynthetic and conjugating functions of the liver, as well as impaired hepatic circulation of bile acids. As a result, feces may be noted in these animals. As a result of studying the bile acid spectrum of bile in ill calves, an increase in the content of almost all bile acids is noted, which is consistent with a significantly high level in the blood on the 30th day of life. The regularities determined indicate the development of intrahepatic cholestasis in the sick calves.
At the same time, with the additional inclusion of the reparative action based on milk phospholipids in experimental animals of the «FLP-MD» dietary supplement in the traditional therapeutic regimen, the recovery of bile-producing liver function and hepatic circulation of bile acids is significantly improved. Peculiarities of the intermediate exchange of bile acids in the body of calves that have had functional enteropathology are revealed, indicate the need for correction of established changes and acceleration of their recovery, it is possible to implement when reparative drugs are included in traditional treatment regimens, in particular dietary supplements «FLP-MD» based on phospholipids milk.
Keywords: bile acids, blood, bile, calves, enteropathology, reparative therapy, milk phospholipids
References
A.s. 4411066/14 SSSR, MBI G 01 N 33/50. A method of determining bile acids in biological fluids / Veselsкiy, S. P., Lyashchenко, P. S., & Luкyanenко I. А. (SSSR). - Publ. 30.01.1991, Byul. N 4. (in Ukrainian)
Björnsson, E. S., & Hoofnagle, J. H. (2016). Categorization of drugs implicated in causing liver injury: critical assessment based on published case reports. Hepatology. 63(2):590-603.
https://doi.org/10.1002/hep.28323
Blas-Garcia, A., Apostolova, N., Valls-Belles, V., & Esplugues, J. V. (2016). Endoplasmic reticulum and mitochondria: independent roles and crosstalk in fatty liver diseases and hepatic inflammation. Curr. Pharm. Des. 22(18):2607-2618.
https://doi.org/10.2174/1381612822666160204120354
Calitz, C., Hamman, J. H., Fey, S. J., Wrzesinski, K., & Gouws, C. (2018). Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective. Toxicology Mechanisms and Methods. 28(5), 369-385.
https://doi.org/10.1080/15376516.2017.1422580
Cole, H. L., Pennycook, S., & Hayes, P. C. (2016). The impact of proton pump inhibitor therapy on patients with liver disease. Alimentary Pharmacology & Therapeutic, 44(11-12):1213-1223.
https://doi.org/10.1111/apt.13827
Gryschenko, V. A., & Litvinenko, O. N. (2007). Osoblyvosti zhovchno-kyslotnogo spectra mihurovoi zhovchi ta duodynalnogo vmistu v myshei pry medykamentoznomu gepatyti i zastosuvanni koreguiuchoi terapii Peculiarities of bile-acid spectrum of cystic bile and duodenal contents in mice with medical hepatitis and applying remedial therapy. Ukrainskii Biohimicheskii Zhurnal - Ukrainian Biochemical Journal. 79(4):97-101 (in Ukrainian).
Gryschenko, V. A., Veselsky, S. P., & Litvinenko, O. N. (2008). Kontsentratsiia zhovchnyh kyslot u vmisti porozhnioi kyshky ta kali schuriv pry medykamentoznomu gepatyti i zastosuvanni koreguiuchoi terapii. The concentration of bile acids in the jejunum contents and rats' feces with medical hepatitis and applying remedial therapy. Vet. med. Ukrainy - Veterinary Medicine of Ukraine. 6:14-16 (in Ukrainian).
Melnychuk, D. O., Tomchuk, V. A., Yanchuk, P. I., Gryshchenko, V. A., Reshetnyk, E. M., Synelnyk, T. B., Capenko, P. K., Kartyfuzova, Zh. V., Govorukha, T. M., Makarchuk, M. Yu., & Veselskyj, S. P. (2015). Research methods of liver and biliar system functional state. NUBiP Ukraine, Kyiv, 414 p. (in Ukrainian).
Pаt. 78306 UA, A 61K 35/20. Veterinary biologically active additive and method of reparative therapy in dyspepsia of newborn calves / Melnychuk, D. O., & Gryshchenko, V. A. - Publ. 15.03.2007, Bul. N 3 (in Ukrainian).
Rui, L. (2014). Energy metabolism in the liver. Comprehensive Physiology. 4(1):177-197.
https://doi.org/10.1002/cphy.c130024
Rykalo, N. A., & Yarovenko, L. A. (2015). Features of reparative regeneration of the liver tissue in rats during experimental tetrachloromethanic and alcoholic hepatitis. Pathologia, 1(33):84-89 (in Ukrainian).
https://doi.org/10.14739/2310-1237.2015.1.42934
Stremoukhov, O. O. (2013). Effect of bile acids on digestion. Original research. 81(6):47-49.
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