OBTAINMENT SPECIFIC IMMUNE SERA TO TESHO- AND ENTEROVIRUS OF PIGS ON RABBITS
DOI:
https://doi.org/10.31548/ujvs2019.04.001Keywords:
специфічні імунні сироватки, тешовіруси, ентеровіруси, схема імунізаціїAbstract
Abstract. The identification of porcine Tesho- and Enteroviruses and the study of their antigenic properties requires the presence of high quality specific immune sera.
The aim of our studies was to obtain specific immune sera for porcine Tesho- and Enteroviruses by short scheme of the immunization of rabbit with two adjuvants for comparison. Five reference strains of porcine Tesho- and Enteroviruses were used as antigens (Bereznyansky, Talfan, Т-80, F 7 and K 9). Three reduced regimens of the immunization of the rabbits have been applied. They provided two, three, and four times the number of injections with a three-day interval between them.
The adjuvant MONTANIDE ISA-25 (Seppic, France) was used to reduce the occurrence of complications in the organism of the animal compared to the lanolin-petrolatum mixture. The highest titers of virus neutralizing antibodies in the specific immune sera 10.33 ± 0.21 – 11.33 ± 0.21 log2 were received by us with four antigen administration at three-day intervals with the adjuvant МОNTANIDE ISA-25.
The use of the adjuvant of MONTANIDE ISA-25 facilitates the introduction of antigen blends, does not cause complications and promotes the activity of immune sera. The new rabbit immunization scheme, which provides four times the antigen injection at three-day intervals, provides specific antibodies in high titers.
Keywords: specific immune sera, teshoviruses, enteroviruses, immunization scheme
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