ALLERGIC EFFECT OF THE PREGRATE "ZG-2011", CONSISTING OF EXOTOXINS

Authors

  • Y. M. Yanenko State Center for Innovative Biotechnologies, Kyiv
  • G. A. Zaviriuha State Center for Innovative Biotechnologies, Kyiv
  • N. Y. Kos'yanchuk National University of Life and Environmental Sciences of Ukraine

DOI:

https://doi.org/10.31548/ujvs2019.04.011

Abstract

Abstract. Gel-electrophoresis is included in the quality control procedures of protein preparations of pharmacological use in accordance with the regulatory framework of Ukraine and the standards of the European Union and the USA.  Determination of the biological activity of the drug is prescribed at the stage of development of the active substance and the drug as amended by the order of the Ministry of Health of Ukraine dated January 4, 2013 No. 3, registered with the Ministry of Justice of Ukraine on March 15, 2013 No. 425/22957.

Goal: to study the exotoxins of pathogenic microorganisms on the content of total protein by the electrophoretic method, which are part of the experimental drug "ZG-2011", a study of the allergenic effect of the drug.

The amount of protein was determined spectrophotometrically using the Pierce BCA Protein Assay Kit (Thermo scientific).  The study of the allergenic effect of the drug "ZG-2011" was carried out in a delayed-type hypersensitivity reaction in mice.

Research methods: method of counting colonies of organisms, determination of the concentration titer in the reaction of disk precipitation, protein studies using electrophoresis.

Results: the quantitative protein content in exotoxins of strains of Bacillus anthracis was established according to the study.  The exotoxin of the strain Bacillus anthracis K-79Z, in the titer of specific anthrax proteins 1: 128, has a low amount of total protein.  While the exotoxins of the vaccine strains of B. anthracis 55, B. anthracis SB at various titers has a stable high rate.  A study of the allergenic effect of the drug that contains exotoxins shows that the administration of the drug leads to an increase in the delayed-type hypersensitivity reaction index in mice of both sexes.  It was found that the drug is able to cause a general anaphylaxis reaction of weak and moderate intensity in the presence of an individual sensitivity of the body to its components.  A study of the influence of exotoxins on laboratory animals and preparations based on exotoxins is expected in the future.

Keywords: allergenic effect, exotoxin, electrophoresis, anthrax

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Published

2019-12-17

Issue

Vol. 10 No. 4 (2019)

Section

Article

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