MORPHOLOGICAL COMPOSITION, ACID-SENSITIVE EQUILIBRIUM AND AQUEOUS SPECTRUM OF BLOOD OF GREAT GREAT LITTLE ART OF DIFFERENT AGE OF FOREIGN EXCLUSION
DOI:
https://doi.org/10.31548/ujvs2019.03.012Abstract
Studies have shown that newborn calves and animals 3 and 6 months of age are higher than pulse rates compared to heifers at the age of 12 months and dry cows based on steady body temperature and morphological parameters of blood. It was shown that for newborn calves the state of subcompensated respiratory acidosis is characteristic in contrast to calves of 3 months of age, and for an animal 6 and 12 months of age of subcompensated metabolic acidosis, characterized by low levels of blood counts of hydrocarbonates, partial pressure of CO2, total carbon dioxide and from ' the capacitance value of the displacement index of the buffer bases. In the blood of newborn calves, compared to other animal groups, glucose levels are higher than in other blood groups, and in plasma plasma is the calcium and alkaline phosphatase activity, while the total protein, lipids and urea are lower, while the activity of alanine aminotransferase, aspartate aminotransferase, and level of inorganic phosphorus has not changed. Proteins with a molecular weight varying from 35 to 900 kDa are found in blood of calves and heifers of different ages, and the main ones are albumins (24.5-40.7 %), immunoglobulins A and G (11.5-30.5 %), transferrin (10.2-15.3 %), fibrinogen (2.4-3.2 %), β-lipoproteins and IgM (1.1-1.8 %), ceruloplasmin (1.2-1.9 % ) and prealbumin (3.4-4.6 %). In the blood plasma of newborn calves, the level of immunoglobulins A i G is 2.2 times lower, 37% is the content of hapthoglobin, 41 % is plasmin, but higher by 34 % of ceruloplasmin, and transferrin substances with a molecular weight of 78 and 72 kDa are higher and 75 kDa - less, whereas other proteins did not differ from animals of 3 months of age. In the 12 month-old heifers, the fractional protein composition was higher in albumin, and lower prealbumin levels, which were also lower in cows during the dry period.
Keywords: calves, heifers, cows, blood, proteins
References
Barabash, V. I., Petrenko, V. I., Loza, A. A. ed.(1999). Zdatnist holshtynskoi khudoby do adaptatsii v umovakh Prydniprovia. [Ability of Holstein cattle to adapt in the conditions of Prydniprovia]. Naukovyi visnyk Lvivskoi derzhavnoi akademii veterynarnoi medytsyny im. S. Z. Hzhytskoho. [Scientific herald of the Lviv State Academy of Veterinary Medicine. SZ Gzhytsky]. part 2, 152-155.[in Russian].
Chumachenko, V. E, Vыsotskyi, A. M, Serdiuk, N. A, Chumachenko, V. V. (1990). Opredelenye estestvennoi rezystentnosty y obmena veshchestv u selskokhoziaistvennыkh zhyvotnыkh. [Determination of natural resistance and metabolism in farm animals.]. Kyiv: Urozhai. Р.[in Russian].
Eremenko, V. Y., Popova, E. L., Stuzhnaia, T. A. (2014). Belkovыi spektr krovy u korov s raznoi molochnoi produktyvnostiu. [Cold Protein spectrum of blood in cows with different milk production]. Vestnyk Kurskoi hosudarstvennoi selkhozakademyy. [Bulletin of the Kursk State Agricultural Academy]. № 7. 69-70. [in Russian].
Espejo, L. A., Endres, M. I., Salfer, I. A. (2006). Poshyrenist kulhavosti u vysokoproduktyvnykh holshtynskykh koriv, rozmishchenykh u naisvizhishii sarai v Minnesoti [Prevalence of lameness in high - producing Holstein cows housed in freest all barn in Minnesota]. Journal Dairy Science. 247 № 89 (8). 3052-3058. [in Еnglish].
https://doi.org/10.3168/jds.S0022-0302(06)72579-6
Havryliuk, O. O. (2017). Vplyv mikroklimatu korivnyka pry riznykh sposobakh utrymannia na yakist moloka koriv. [Influence of the microclimate of the cow's milk in different ways of maintaining the quality of milk of cows]. Visnyk Sumskoho NAU. [Visnyk of Sumy NAU].(31). 48-50. [in Ukraine].
Kamиshnykov, V. S. (2000). Spravochnyk po klynyko-byokhymycheskoi laboratornoi dyahnostyke. [Handbook of clinical and biochemical laboratory diagnostics.]. Mynsk: Belarus, 463.[in Russian].
Kandyba, V. M, Ibatullin, I. I, Kostenko, V. I. (2012). Teoriia i praktyka normovanoi hodivli VRKh. [Theory and practice of normalized feeding of cattle]. Zhytomyr: PP «Ruta» 860[in Ukraine].
Kokunin, V. A. (1975). Statystychna obrobka danykh z nevelykoiu kilkistiu vyprobuvan. Ukrainskyi biokhimichnyi zhurnal. [Statistical data processing with a small number of trials. Ukrainian biochemical journal]. 776-790.[in Еnglish].
Korol, A. P. (2005). Povedinka koriv v umovakh bezpryviazno-boksovoho utrymannia. [Behavior of cows in conditions of seamless-box content]. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii. [The Bulletin of the Poltava State Agrarian Academy].139-142. [in Ukraine].
Kostenko, V. I,. Banias, Yu. Yu. (2011). Vplyv komfortu utrymannia koriv na riven yikh molochnoi produktyvnosti. [Influence of comfort of maintenance of cows on level of their milk productivity]. Problemy zooinzhenerii ta veterynarnoi medytsyny. Zbirnyk naukovykh prats Kharkivskoi DZVA. [Problems of zoinengineering and veterinary medicine. Collection of scientific works of Kharkiv DZVA]. 567-571. [in Ukraine].
Laemmly, U. K. (1970). Rozshcheplennia strukturnykh bilkiv pry zbirtsi akuratnoho bakteriofahaT4. Pryroda. [Cleavage of structural proteins during the assembly of the neat of bacteriophage T4. Nature].680-685.[in Еnglish].
https://doi.org/10.1038/227680a0
Levchenko, V. I., Vlizlo, V. V., Kondrakhin, I. P. ed. (2017). Klinichna diahnostyka khvorob tvaryn. [Clinical diagnostics of animal diseases.]. Bila Tserkva. 544.[in Ukraine].
Shandrenko, S. G., Golovin, A. S., Dimitrenko, M. P., Yurchenko, A. I. (2003). Kompiuterna reiestratsiia ta analiz TLC. Zhurnal khromatohrafii suspilstva.[The computer registration and analysis of TLC. Journal of chromatography of the society]. 22-30.[in Еnglish].
Shkurlo, T. P. (2017). Povedinka vysokoproduktyvnykh koriv uzymku za bezpryviazno-boksovoho utrymannia. [Behavior of high-yielding cows in winter for unbounded-box content]. Visnyk ahrarnoi nauky. [Bulletin of Agrarian Science]. 37-40. [in Ukraine].
https://doi.org/10.31073/agrovisnyk201708-06
Tiupina, N. V. (2013). Porivnialna otsinka pryrodnoi rezystentnosti teliat otrymanykh vid koriv holshtynskoi porody za riznykh tekhnolohii yikh vykorystannia ta umov utrymannia. [Comparative estimation of natural resistance of calves obtained from Holstein breed cows for different technologies of their use and conditions of retention]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny i biotekhnolohii im. S.Z. Hzhytskoho. [Scientific herald of the Lviv National University of Veterinary Medicine and Biotechnology. SZ Gzhytsky]. Vol.15 №3 (57), 427-431. [in Ukraine].
Tomin, Ye. F., Zakharenko, M. O. (2011). Povedinka ta kyslotno-luzhnyi stan krovi vysokoproduktyvnykh laktuiuchykh koriv zarubizhnoi selektsii za dii vysokykh temperatur povitria. [Behavior and acid-alkaline state of blood of high-yielding lactating cows of foreign selection for the effects of high air temperatures]. Naukovyi visnyk NUBiP Ukrainy. [Scientific herald of NUBiP of Ukraine]. 184-189. [in Ukraine].
Varpikhovskyi, R. L. (2017). Povedinkovi reaktsii neteliv za bezpryviazno-boksovoho utrymannia u modulno-hrupovii klittsi. [Behavioral reactions of non-cells for unbounded-box retention in a modular-group cell]. Ahrarna nauka ta kharchovi tekhnolohii. Naukovyi visnyk Vinnytskoho VNAU. [Agricultural science and food technologies. Scientific Herald of Vinnytsia State University].1 (95).113-120. [in Ukraine].
Vlizlo, V. V., Fedorchuk, R. S., Ratych, I. B. (Vlizlo V. V., 2012).. Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni: Dovidnyk. [Laboratory methods of research in biology, livestock and veterinary medicine: Directory.]. Lviv: SPOLOM, 764.[in Ukraine].
Volhyn, V. Y., Romanenko, L. V., Fёdorova, Z. L. (2010). Sovershenstvovanye byokhymycheskykh sposobov kontrolia polnotsennosty kormlenyia vusokoproduktyvnukh korov. [Improving biochemical methods to control the usefulness of feeding highly productive cows.]. Zootekhnyia, 2,10-11. [in Russian].
Zakharenko, M. O. (2017). Fraktsiinyi sklad bilkiv plazmy krovi laktuiuchykh koriv. [The fractional composition of blood plasma proteins of lactating cows]. Naukovi dopovidi NUBiP Ukrainy. [Scientific reports of NUBiP of Ukraine.]. № 5 (69). [in Ukraine].
Zubets, M. V., Tokarev, N. F., Vynnychuk, D. T. (1996). Etolohyia krupnoho rohatoho skota. [Ethology of cattle]. Kyiv: Ahrarna nauka, 213.[in Ukraine].
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