Effect of egg storage period on blastoderm cell viability, embryonic development rate and incubation results

Authors

  • N. V. Shomina , Державна дослідна станція птахівництва Національної академії аграрних наук України, с. Бірки. Харківська область
  • O. M. Baidevlyatova , Державна дослідна станція птахівництва Національної академії аграрних наук України, с. Бірки. Харківська область

Keywords:

viability of blastoderm cells, intensity of embryo development, duration of hatching period, hatching energy, egg hatchability

Abstract

The article analyzes the relationship between the level of blastoderm cells death during storage and the hatchability of eggs. Changes in the incubation parameters of eggs, the intensity of embryo development, the duration of the hatching period, the energy of chick's hatching depending on the period of egg storage were observed. It is known that when an egg is laid, the chicken embryo is represented by a multilayered blastoderm. During egg storage, the total number of blastoderm cells decreases, which further affects the viability of the embryo, the hatchability and chick quality. The aim of the study was to investigate the effect of the duration of egg storage period on blastoderm cells death and to establish the relationship between this indicator and the results of incubation. The work was carried out at State Poultry Research Station of NAAS on hatching eggs of red Rhode Island chickens. The study of the viability of blastoderm cells, the study of the intensity of embryo development, the duration of the hatching period, the energy of hatching were carried out in groups of eggs with storage period of 2 (control group), 10, 14, 17 and 21 days. It was found that with increasingof egg storage duration, there were significant decrease in the number of viable blastodermal cells (from 95.0±1.3% in the control group to 60.0±2.8% in the group of eggs after three weeks of storage), which negatively affected hatchability of eggs (r=0.99). The egg hatchability in the group after three weeks of storage was 57.0±3.1%, which is 28.1% less than in the control. Long duration of the egg storage period directly and indirectl(due to the inconsistency of the standard incubation regime with the needs of underdeveloped embryos) influenced the intensity of embryo development, duration of hatching period and hatching energy, which negatively affected the quality of chickens.

References

Breslavets, V.A., Shomina, N.V., Artemenko, A.B., & Baydevlyatova, O.N. (2018). Inkubatsiya yaits selskohozyaystvennoy ptitsyi. Metodicheskoe posobie. Borki. [Incubation of poultry eggs]. Metodicheskoe posobie [Toolkit]. Borki, 126. [in Russian].

Dyadichkina, L.F., Pozdnyakova, N.S., Glavatskih, O.V, Melehina, T.A., Tishenkov, A.N., Zaytseva, N.P., Hrebtova, E.A., & Rebrakova, T.M. (2004). Rukovodstvo po biologicheskomu kontrolyu pri inkubatsii yaits selskohozyaystvennoy ptitsyi [Guidelines for biological control during incubation of eggs of poultry]. Metodicheskie rekomendatsii [Guidelines]. Sergiev-Posad: VNITIP, 35-41. [in Russian].

Petuhov, V.L., Zhigachev, A.I., & Nazarova, G.A. (1985). Veterinarnaya genetika s osnovami variatsionnoy statistiki [Veterinary genetics with the basics of variation statistics]. Moskva: Agropromizdat, 1985, 150-153. [in Russian].

Addo, A., Hamidu, J.A., Ansah, A.Y., & Adomako, K. (2018). Impact of Egg Storage Duration and Temperature on Egg Quality, Fertility, Hatchability and Chick Quality in Naked Neck Chickens. International Journal of Poultry Science. 17(4), 175-183. https://doi.org/10.3923/ijps.2018.175.183

Arora, K. L., & Kosin, I. L. (1966). Changes in the gross morphological appearance of chicken and turkey blastoderms during pre-incubation storage. Poultry Science, 45, 819-825. [in English]. https://doi.org/10.3382/ps.0450819

Bakst, M.R., Akuffo, V., Nicholson D., & French, N. (2012.) Comparison of blastoderm traits from 2 lines of broilers before and after egg storage and incubation. Poultry Science, 91, 2645-2648. https://doi.org/10.3382/ps.2011-02118

Bloom, S. E., Muscarella, D. E., Lee, M. Y., & Rachlinski, M. (1998). Cell death in the avian blastoderm: resistance to stress-induced apoptosis and expression of anti-apoptotic genes. Cell Death Differentiation, 5, 529-538. [in English]. https://doi.org/10.1038/sj.cdd.4400381

Hamidu, J.A., Adomako, K., Adamu, S., Ajaottor, A.M., Amankwah, G.J., Gyasi, K.M., Shiburah, E.M., Darko, J.O. & Konadu L.A. (2018). Impact of layer egg storage conditions on eggs and embryo quality indices prior to incubation. Livestock Research for Rural Development, 30(11). http://www.lrrd.org/lrrd30/11/cont3011.htm. [in English].

Hamidu, J. A., Rieger, A. M., Fasenko, G. M., & Barreda, D. R. (2010). Dissection of chicken blastoderm for examination of apoptosis and necrosis by flow cytometry. Poultry Science, 89, 901-909. http://citeseerx.ist.psu.edu/viewdoc/download? https://doi.org/10.3382/ps.2009-00552

Reijrink, I. A. M., Meijerhof, R., Kemp B., & van den Brand H. (2010). Influence of egg warming during storage and hypercapnic incubation on egg characteristics, embryo development, hatchability, and chick quality. Poultry Science, 89, 2470-2483. https://pubmed.ncbi.nlm.nih.gov/20952711/. [in English].

https://doi.org/10.3382/ps.2010-00798

Reijrink, I. A. M., Meijerhof, R., Kemp, B., & van den Brand H. (2008). The chicken embryo and its micro environmemt during egg storage and early incubation. World's Poultry Science Journal, 64, 581-598. [in English]. https://doi.org/10.1017/S0043933908000214

Yassin, H., Velthius, A.G.J., Boerjan, M., & van Riel, J. (2009). Field study on broilers first-week mortality. Poultry Science, 88, 798-804. https://pubmed.ncbi.nlm.nih.gov/19276423/. [in English].

https://doi.org/10.3382/ps.2008-00292

Published

2021-11-24

Issue

Section

Incubation