Hematological and biochemical indicators of laboratory rats using probiotic bacteria V. Licheniformis and V. Subtilis
DOI:
https://doi.org/10.31548/poultry2022.07-08.020Keywords:
water, filters, B. subtilis, B. licheniformis, ratsAbstract
The search of new methods for water teatment a ety for both humans and animals (including a bird) is an urgent and important problem today. The purpose of the work is to investigate the effect of drinking water with the use of probiotic bacteria B. licheniformis and B. subtilis on the rats. Experiments were conducted on three-month-old male non-linear laboratory rats with an average body weight of 153.9±2.4 g. There were 15 rats each in the experimental and control groups. For 3 months, the rats of the control group were fed with water taken from a well ("buvette"), the rats of the experimental group were fed with the same water, but passed through a filter with immobilized probiotic bacteria Bacillus licheniformis UKM-5514 and Bacillus subtilis UKM B-5007. Probiotic bacteria have been found to suppress other water microbiota. The positive effect of drinking water with probiotic bacteria in rats was established. In the rats of the research group, already after 1 month of drinking water with probiotic bacteria, the appetite and the condition of the fur, which became thicker and more shiny, compared to the control, improved. An increase in their mass was noted in the experimental group after 2 months by 9%, after 3 – by 12% compared to the control. The content of hemoglobin, total protein and alkaline phosphatase increased in experimental rats. According to the results of pathologicalanatomical and histological studies, the control and experimental animals did not differ from each other. Scientific novelty. A "Ukrainian modification" of "English filters" is proposed, which consists in creating a biofilm of probiotic bacteria on sludge (sand, coal, etc.). This method of water treatment rids it of existing microorganisms and enriches the filtered water with probiotic bacteria, which can be used in animal husbandry, poultry farming, and for the purification of tap drinking water.
References
Abu Hasan, H., Muhammad, M. H., & Ismail, N. I. (2020). A review of biological drinking water treatment technologies for contaminants removal from polluted water resources. Journal of Water Process Engineering, 33. doi:10.1016/j.jwpe.2019.101035 [in English].
Arenas, L. R., Gentile, S. R., Zimmermann, S., & Stoll, S. (2022). Fate and removal efficiency of polystyrene nanoplastics in a pilot drinking water treatment plant. Science of the Total Environment, 813. doi:10.1016/j.scitotenv.2021.152623 [in English].
Bayer, E.V., Novozhitskaya, Yu. N., Shevchenko, L. B., & Mykhalska, V. M. (2017а). Monitorynh zalyshkiv veterynarnykh preparativ u kharchovykh produktakh [Monitoring of residues of veterinary preparations in food products]. Ukrainian Journal of Ecology, 7 (3), 251-257. doi:10.15421/2017_76 [in Ukrainian].
Bayer, E. V., Novozhitskaya, Yu. N., Shevchenko, L. V., & Mykhalska, V. M. (2017b). Vyznachennia vmistu antybiotykiv ta sulfanilamidnykh preparativ u molotsi skryninhovym metodom [Determination of the content of antibiotics and sulfanilamide drugs in milk by screening method]. Ukrainian Journal of Ecology, 7(4), 576-582.
Ghosh, B. K., & Ghosh, N. N. (2018). Applications of Metal Nanoparticles as Catalysts in Cleaning Dyes Containing Industrial Effluents: A Review. Journal of Nanoscience and Nanotechnology, 18(6), 3735-3758. doi:10.1166/jnn.2018.15345 [in English].
Hu, J. J., Zhao, Y. X., Yang, W. L., Wang, J. Q., Liu, H., Zheng, P., & Hu, B. L. (2020). Surface ammonium loading rate shifts ammonia-oxidizing communities in surface water-fed rapid sand filters. Fems Microbiology Ecology, 96(10). doi:10.1093/femsec/fiaa179 [in English].
Jeon, Y., Li, L., Calvillo, J., Ryu, H., Domingo, J. W. S., Choi, O., & Seo, Y. (2020). Impact of algal organic matter on the performance, cyanotoxin removal, and bio fi lms of biologically-active fi ltration systems. Water Research, 184. doi:10.1016/j.watres.2020.116120 [in English].
Kumar, P., Hegde, K., Brar, S. K., Cledon, M., Kermanshahi-pour, A., Roy-Lachapelle, A., & Galvez-Cloutier, R. (2020). Co-culturing of native bacteria from drinking water treatment plant with known degraders to accelerate microcystin-LR removal using biofilter. Chemical Engineering Journal, 383. doi:10.1016/j. cej.2019.123090 [in English].
Mohammadi-Barzelighi, H., Nasr-Esfahani, B., Bakhshi, B., Daraei, B., Moghim, S., & Fazeli, H. (2019). Analysis of antibacterial and antibiofilm activity of purified recombinant Azurin from Pseudomonas aeruginosa. Iranian Journal of Microbiology, 11(2), 166-176. [in English].
Ma, B., LaPara, T. M., & Hozalski, R. M. (2020). Microbiome of Drinking Water Biofilters is Influenced by Environmental Factors and Engineering Decisions but has Little Influence on the Microbiome of the Filtrate. Environmental Science & Technology, 54(18), 11526-11535. doi:10.1021/acs.est.0c01730 [in English].
Vu, C. T., & Wu, T. T. (2022). Enhanced Slow Sand Filtration for the Removal of Micropollutants from Groundwater. Science of the Total Environment, 809. doi:10.1016/j.scitotenv.2021.152161 [in English].
Sharma, D., Taylor-Edmonds, L., & Andrews, R. C. (2018). Comparative assessment of ceramic media for drinking water biofiltration. Water Research, 128, 1-9. doi:10.1016/j.watres.2017.10.019 [in English].
Xu, L. K., Campos, L. C., Canales, M., & Ciric, L. (2020). Drinking water biofiltra genes and the association with bacterial community. Water Research, 182. doi:10.1016/j.watres.2020.115954 [in English].
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