Sterile culture of Camelina Sativa (L.) Crantz Var. "Klondike": features of obtaining and multiplication in vitro

Authors

  • R. Ivannikov M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine
  • V. Anishchenko L.M. Litvinenko Institute of Physical-Organic Chemistry and Coal Chemistry of NAS of Ukraine
  • I. Laguta Chuiko Institute of Surface Chemistry of NAS of Ukraine
  • P. Kuzema Chuiko Institute of Surface Chemistry of NAS of Ukraine
  • N. Smirnova Chuiko Institute of Surface Chemistry of NAS of Ukraine
  • O. Stavinskaya Chuiko Institute of Surface Chemistry of NAS of Ukraine

Abstract

Abstract. The vast majority of human energy needs are met by fossil fuels. In the short term, the use of non-renewable resources will not be able to contribute to the sustainable development of the world economy due to depletion of their reserves, rising oil prices and significant damage to the environment. These environmental and economic risks are the basis for the development of renewable bioenergy. Oilseeds are considered as a promising source for biofuel production. One of the areas of work in the range of outlined issues is the selection, selection and development of biotechnology of promising oilseeds, including red seed Camelina sativa (L.) Crantz.

The text of the article highlights the methodological issues of obtaining a sterile culture of ryegrass varieties of Ukrainian selection "Klondike" and the development of procedures for its further multiplication in vitro. Classical morphological and biotechnological methods of obtaining and maintaining sterile crops were used in the study tasks.

Aseptic culture of ryegrass was obtained from zygotic seed germs. We optimized the sterilization procedure of plant material and for the first time showed a positive effect on the disinfection of sterile-type sterilants (0.01%, Merck, exposure 15-20 min) and chlorhexidine bigluconate (0.05%, exposure 30-35 min).

It was established that Murashige-Skug (MS) is the optimal formulation of the basic medium for the experimental variety of red rice at the initial stages of introduction, and later, during clonal micropropagation. The best biomass growth rates were recorded on MS with sucrose content in the range of 20 g/l.

Thus, we developed a decontamination procedure and obtained a sterile culture of red rice, selected media for introduction and subsequent multiplication in order to increase plant biomass in vitro.

The results obtained during the work are part of a comprehensive study and will be used in the development of eco-innovative approaches to the efficient use of renewable plant resources through photocatalytic conversion of biomass.

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Published

2022-06-20

Issue

Vol. 12 No. 4 (2021)

Section

Статті

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