FATTY ACID IN LIPID OF CAT BONE MARROW MESENCHIMAL STEM CELLS
DOI:
https://doi.org/10.31548/dopovidi2016.04.025Keywords:
mesenchymal stem cells, bone marrow, cat, saturated, unsaturated fatty acidsAbstract
Defined lipids fatty acids composition of cat bone marrow mesenchymal stem cells. Mesenchymal stem cells (MSCs) obtained from cat bone marrow. The process of culturing MSCs cat was performed in CO2 incubator at 5% CO2 and temperature of 37 °C in DMEM medium with the addition of 1% antibiotic-antimycotics and 15-20% fetal bovine serum. When the monolayer confluency was 70-90%, cells removed and carried subсultivation 3-4 times to reduce the heterogeneity of cells culture. To determine the fatty acid composition of lipids MSCs used 4-th passage.
For research content of fatty acids in lipids cat MSCs was performed by the method of gas-liquid chromatography. Bone marrow stem cells in cat contain lipids short-, medium- and longchain fatty acids. In the stem cells of the cat bone marrow was found 18 fatty acids, in saturated – most quantity of palmitic acid – 32.4 , in monounsaturated - oleic acid – 23.15, in polyunsaturated – linoleic acid – 8.51% . The smallest quantity in the cells was found cis-8,11,14-eykozatriyenic acid –0,01%. The total amount of saturated fatty acids in MSCs was 64,88, unsaturated fatty acids – 35,12%. Monoyenic fatty acids identified in the number of 25,71, and polyenes - 9,41%. Saturation index – 1,85. The index value n3 fatty acids to n6 MSC cat is 0,08.
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