FEATURES OF PREPARATION OF SAMPLES FOR DIAGNOSTICS OF BOVINE LEUKOSISUSING THE IMMUNE BIOSENSOR
DOI:
https://doi.org/10.31548/biologiya2018.287.136Abstract
The article discusses the best options for the preparation of blood and milk samples for the purpose of diagnosing retroviral enzootic bovine leukosis in cattle and assessing the origin of dairy products from sick or healthy animals. The effectiveness of using samples in the native state and after their processing with the removal of red blood cells, their membranes, the fat component and casein were analyzed. It has been established that when performing screening analysis it can be carried out using whole blood and milk samples. This is especially possible in the case of diagnosis of the disease at the stage of its progressive development. The use of such samples significantly reduces analysis time and makes it simpler. But if it is necessary to carry out data verification and detection of cattle retroviral leukemia virus already in the early periods, then, in accordance with the results obtained by us, samples prepared in the form of blood serum or milk serum should be used. The latter should also be used to accurately assess the origin of milk, especially when it is necessary to control its quality when collecting from several animals, among which may be sick individuals, as well as in the early stages of the development of the pathological process.
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