INFLUENCE OF GIBERELIN PRIMING ON GROWTH AND CONTENT OF SOLUBLE CARBOHYDRATES IN SEEDLING OF SOFT WHEAT UNDER VERNALIZATION

Authors

  • V.V. Chumakova
  • O.O. Avksentiyeva

DOI:

https://doi.org/10.31548/biologiya2018.287.173

Abstract

The present study was designed to investigate the influence of gibberellin (GA) priming under vernalization on the growth response, content and fractional composition of soluble carbohydrates in seedlings of 3 varieties of winter wheat - Doridna, Statna and Alians. Modeling different levels of trophic support of the vernalization process and investigating the influence of GA, the following variants were used in the experiments: 1) grains + water (control, optimal trophic support); 2) grains + 10 mg / l GA3, (optimal trophic support); 3) isolated embryos + water (control, lack of trophic support); 4) isolated embryos + 10 mg / l GA3 (lack of trophic support). Vernalization was carried out during 45 days at 4 ° C in Petri dishes on wet filter paper. According to the results, the stimulation of growth processes was identified under the GA effect in all investigated winter wheat varieties. The stimulation occurred as linear growth and biomass accumulation of seedlings, the intensity of which depended on the level of trophic support of these processes. The GA priming in the variant with the lack of trophic support did not significantly stimulate the content of carbohydrates in the varieties Doridna and Statna. It noticeably stimulated the content of carbohydrates in the Alians variety due to changes of oligosaccharides content. The patterns of phytohormonal and trophic regulation of vernalization processes in winter wheat are discussed.

References

Dennis, E., Peacock, W. (2009). Vernalization in cereals. Journal of Biology, 8, (57), 1–4. doi.org/10.1186/jbiol156.

Avksentyeva, O. A., Zhmurko, V. V. (2011). Fiziologiya tsveteniya [Physiology of flowering]. Kharkiv, Ukraine: V.N. Karazin KhNU, 130.

Aoki, N., Scofield, G. N., Wang, X.-D., Offler, C. E., Patrick, J. W., Furbank, R. T. (2006). Pathway of Sugar Transport in Germinating Wheat Seeds. Plant Physiology, 141 (4), 1255–1263. doi: 10.1104/pp.106.082719.

Koch, K. (2004). Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Current Opinion in Plant Biology, 7 (3), 235–246. doi: 10.1016/j.pbi.2004.03.014.

Shang, M., Wang, X., Zhang, J., Qi, X., Ping, A., Hou, L., Xing, G., Li, G. and Li, M. (2017). Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp. Chinensis Makino). Front. Plant Sci., 8, 1533. doi: 10.3389/fpls.2017.01533.

Pearce, S., Vanzetti, L.S., Dubcovsky J. (2013). Exogenous Gibberellins Induce Wheat Spike Development under Short Days Only in the Presence of VERNALIZATION1. Plant Physiology, 163, 1433-1445. doi: 10.1104/pp.113.225854.

Firuzeh, R., Khavari-Nejad, R. A., Najafi, F., Saadatmand, S. (2015). Effect of Gibberellins on Sugar, Protein and Malondialdehyde Contents in Savory Plant (Satureja hortensis L.) under Salt Stress. J. Appl. Environ. Biol. Sci., 5 (7S), 23-29.

Silva Vieira, M. R., Simoes, A. N., Rocha, A. T., Silva, L. Z., Sousa, P. A. (2013). Relationship between gibberellins and carbohydrates in vegetable products. African Journal of Plant Science, 7 (10), 445-447. doi: 10.5897/ajps2013.1054

Ermakov, A. I., Arasimovich, V. V., M. I., Yarosh, N. P. et al ed. (1987). Metody byokhymycheskoho yssledovanyia rastenyi [Methods for the biochemical analysis of plants]. Lenynhrad: Ahropromyzdat, 430 s.

Avksentyeva, O. A., Shulik, V. V. (2017). Doslidzhennya vplyvu kontrastnykh umov trofichnoho zabezpechennya za yarovyzatsiyi na mitotychnu aktyvnist' merystem, rist ta rozvytok ozymoyi pshenytsi [Research of influence of contrasting trophic conditions of vernalization on the mitotic activity of meristems, growth and development of winter wheat]. ScienceRise: Biological Science, 2 (5), 4–9. doi: 10.15587/2519-8025.2017.99664.

Downloads

Published

2018-11-16

Issue

No. 287 (2018)

Section

Статті

License

Relationship between right holders and users shall be governed by the terms of the license Creative Commons  Attribution – non-commercial – Distribution On Same Conditions 4.0 international (CC BY-NC-SA 4.0):https://creativecommons.org/licenses/by-nc-sa/4.0/deed.uk

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).